Krätzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning W D
Research Laboratories of Schering AG, Berlin, Germany.
Eur J Biochem. 1996 Mar 15;236(3):827-36. doi: 10.1111/j.1432-1033.1996.t01-1-00827.x.
We report the isolation and characterisation of cDNAs encoding three different, human members of the cysteine-rich secretory protein (CRISP) family. The novel CRISP-1 exists in five cDNA subtypes differing by the presence or absence of a stretch coding for a C-terminal cysteine-rich domain so far found in all members of the family, and by the length of their 3'-untranslated region. CRISP-2 cDNA corresponds to the previously described TPX1 form, with so far unreported 5'-untranslated sequence heterogeneities while CRISP-3 cDNA codes for a new, unique protein. Northern blot analysis of various human organs indicates that CRISP-1 transcripts are epididymis-specific whereas CRISP-2/TPX1 transcripts are detected mainly in the testis and also in the epididymis. CRISP-3 transcripts are more widely distributed and found predominantly in the salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. A protein reacting with an anti-mouse CRISP-1 antibody was isolated from human epididymal extracts and N-terminal sequencing revealed that it corresponded to the CRISP-1 cDNA we have isolated. In contrast to findings on its rat counterpart epididymal protein DE/acidic epididymal glycoprotein (AEG), no significant association of CRISP-1 with human spermatozoa was observed.
我们报道了编码富含半胱氨酸分泌蛋白（CRISP）家族三种不同人类成员的cDNA的分离和鉴定。新型CRISP-1存在五种cDNA亚型，它们的差异在于是否存在一个编码C末端富含半胱氨酸结构域的片段（该片段迄今在该家族所有成员中均有发现）以及其3'-非翻译区的长度。CRISP-2 cDNA对应于先前描述的TPX1形式，具有迄今未报道的5'-非翻译序列异质性，而CRISP-3 cDNA编码一种新的独特蛋白质。对各种人类器官的Northern印迹分析表明，CRISP-1转录本是附睾特异性的，而CRISP-2/TPX1转录本主要在睾丸中检测到，在附睾中也有检测到。CRISP-3转录本分布更广泛，主要存在于唾液腺、胰腺和前列腺中，在附睾、卵巢、胸腺和结肠中的含量较少。从人附睾提取物中分离出一种与抗小鼠CRISP-1抗体反应的蛋白质，N末端测序显示它与我们分离的CRISP-1 cDNA相对应。与在大鼠附睾蛋白DE/酸性附睾糖蛋白（AEG）上的发现相反，未观察到CRISP-1与人类精子有明显关联。
