Nanda I, Tanaka T, Schmid M
Department of Human Genetics, University of Würzburg, Germany.
Gene. 1996 May 8;170(2):159-64. doi: 10.1016/0378-1119(95)00828-4.
Chicken ribosomal protein (rp)-encoding genes are currently being studied at the nucleotide level and three independent recombinant phages have been isolated from chicken genomic libraries using cloned cDNA probes. Each of these was shown to include an intron-containing rp gene of chicken (L5, L7a, L37a). In this study the chromosomal location of these three intron-containing rp from the large subunit of the chicken ribosome was determined by fluorescence in situ hybridization. L7a mapped to a microchromosome, whereas L5 and L37a mapped to macrochromosomes 8 and 7, respectively. The results demonstrate that these functionally related genes are widely dispersed in the genome. Furthermore, as in the case of many other evolutionarily advanced eukaryotes, there is no apparent linkage of rp and rRNA genes.
目前正在核苷酸水平上研究鸡核糖体蛋白（rp）编码基因，并且已经使用克隆的cDNA探针从鸡基因组文库中分离出三个独立的重组噬菌体。这些噬菌体中的每一个都显示包含鸡的一个含内含子的rp基因（L5、L7a、L37a）。在本研究中，通过荧光原位杂交确定了来自鸡核糖体大亚基的这三个含内含子的rp基因的染色体定位。L7a定位于一条微染色体，而L5和L37a分别定位于8号和7号大染色体。结果表明，这些功能相关的基因在基因组中广泛分布。此外，与许多其他进化上高等的真核生物一样，rp基因和rRNA基因之间没有明显的连锁关系。
