Valdez-Alarcón J J, Ferrando M, Salerno G, Jimenez-Moraila B, Herrera-Estrella L
Departamento de Ingeniería Genética de Plantas, Centro de Investigación y Estudios Avanzados del I.P.N., Unidad Irapuato, Guanajuato, Argentina.
Gene. 1996 May 8;170(2):217-22. doi: 10.1016/0378-1119(95)00854-3.
A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
分离并测序了一个编码蔗糖磷酸合酶（SPS）的水稻基因组克隆（sps1）。水稻sps1包含13个外显子和12个内含子，一个具有高度有序一级结构的异常长的366bp前导区，以及一个与真核启动子共有序列无明显同源性的启动子区。Southern杂交分析表明，SPS在水稻基因组中由单拷贝基因编码。水稻、玉米、马铃薯和菠菜SPS推导氨基酸（aa）序列的比较表明，这些酶有一个由其前700个aa组成的保守性良好的区域，以及一个可变的C末端区域。水稻sps1表达分析表明，mRNA水平在叶片发育过程中发生变化。在根中未检测到SPS活性和mRNA。
