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来自集胞藻属PCC 6803菌株的蔗糖磷酸合酶：spsA基因的鉴定及在大肠杆菌中表达的该酶的特性分析

Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.

作者信息

Curatti L, Folco E, Desplats P, Abratti G, Limones V, Herrera-Estrella L, Salerno G

机构信息

Centro de Investigaciones Biológicas, Fundación para Investigaciones Biológicas Aplicadas (FIBA) and PROBIOP-CONICET, 7600 Mar del Plata, Argentina.

出版信息

J Bacteriol. 1998 Dec;180(24):6776-9. doi: 10.1128/JB.180.24.6776-6779.1998.

DOI:10.1128/JB.180.24.6776-6779.1998
PMID:9852031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107790/
Abstract

The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.

摘要

报道了单细胞非固氮蓝藻集胞藻PCC 6803中编码蔗糖磷酸合酶（SPS）的原核基因（spsA）的首次鉴定和特性分析。该酶推导的氨基酸序列及一些相关生化特性与植物SPSs的比较显示，在N端和UDP-葡萄糖结合位点区域、底物特异性、分子量、亚基组成及调控特性方面存在重要差异。

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Coarse control of sucrose-phosphate synthase in leaves: Alterations of the kinetic properties in response to the rate of photosynthesis and the accumulation of sucrose.叶片中蔗糖-磷酸合成酶的粗调控制：对光合作用速率和蔗糖积累的响应改变动力学特性。
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