来自集胞藻属PCC 6803菌株的蔗糖磷酸合酶：spsA基因的鉴定及在大肠杆菌中表达的该酶的特性分析
Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
作者信息
Curatti L, Folco E, Desplats P, Abratti G, Limones V, Herrera-Estrella L, Salerno G
机构信息
Centro de Investigaciones Biológicas, Fundación para Investigaciones Biológicas Aplicadas (FIBA) and PROBIOP-CONICET, 7600 Mar del Plata, Argentina.
出版信息
J Bacteriol. 1998 Dec;180(24):6776-9. doi: 10.1128/JB.180.24.6776-6779.1998.
Abstract
The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.
摘要
报道了单细胞非固氮蓝藻集胞藻PCC 6803中编码蔗糖磷酸合酶（SPS）的原核基因（spsA）的首次鉴定和特性分析。该酶推导的氨基酸序列及一些相关生化特性与植物SPSs的比较显示，在N端和UDP-葡萄糖结合位点区域、底物特异性、分子量、亚基组成及调控特性方面存在重要差异。
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