Kurzik-Dumke U, Neubauer M, Debes A
Institut für Genetik, Johannes Gutenberg-Universität, Mainz, Germany.
Gene. 1996 Jun 1;171(2):163-70. doi: 10.1016/0378-1119(95)00885-3.
In this study, we describe the identification of a novel Drosophila melanogaster (Dm) gene, l(2)dtl, characterized by elevated expression under heat-shock (HS) conditions. It encodes a protein of 83 kDa with no homology to known members of the HSP90 family and other proteins. Gene l(2)dtl is located on the right arm of the second chromosome at locus 59F5, close to the tumor suppressor gene l(2)tid, a homolog of the dnaJ encoding a chaperone strongly conserved in evolution. In the following, we present the sequence of l(2)dtl, the putative protein it encodes, and its molecular localization in a closely interspaced gene cluster consisting of at least four nested genes spanning an approximately 10-kb genomic interval. Furthermore, we present the temporal expression of l(2)dtl in the wild type under normal and HS conditions, and describe the isolation and the phenotype of eight embryonic lethal l(2)dtl mutants.
在本研究中，我们描述了一个新的黑腹果蝇（Dm）基因l(2)dtl的鉴定，其特征是在热休克（HS）条件下表达升高。它编码一个83 kDa的蛋白质，与HSP90家族的已知成员及其他蛋白质无同源性。基因l(2)dtl位于第二条染色体右臂的59F5位点，靠近肿瘤抑制基因l(2)tid，l(2)tid是dnaJ的同源物，dnaJ编码一种在进化中高度保守的伴侣蛋白。接下来，我们展示l(2)dtl的序列、它所编码的推定蛋白质，以及它在一个紧密间隔的基因簇中的分子定位，该基因簇由至少四个嵌套基因组成，跨越约10 kb的基因组区间。此外，我们展示了l(2)dtl在野生型正常和热休克条件下的时间表达情况，并描述了八个胚胎致死性l(2)dtl突变体的分离和表型。
