Horvath D H, Watson J B, Travis G H
Department of Psychiatry, UT Southwestern Medical Center, Dallas, TX 75235-9111, USA.
Gene. 1996 Jun 1;171(2):305-6. doi: 10.1016/0378-1119(96)00033-9.
We have tested the gene encoding cortexin, Ctxn, which maps to chromosome 8, as a candidate for the mouse neurological mutants: nervous (nr), tottering (tg) plus tottering-leaner (tgla), and motor neuron degeneration (mnd) by Northern blot analysis of brain poly(A)+ RNA and direct polymerase chain reaction (PCR) sequencing. No difference from wild-type was seen in any of these mutants. Based upon these observations, we conclude that Ctxn is not involved in the genetic defects found in nr, tg or mnd mice.
我们通过对脑 poly(A)+ RNA 进行 Northern 印迹分析以及直接聚合酶链反应（PCR）测序，对定位于 8 号染色体的编码皮质素的基因 Ctxn 进行了检测，将其作为小鼠神经学突变体：神经质（nr）、蹒跚（tg）加蹒跚-倾斜（tgla）以及运动神经元变性（mnd）的候选基因。在这些突变体中均未发现与野生型有差异的情况。基于这些观察结果，我们得出结论，Ctxn 不参与 nr、tg 或 mnd 小鼠中发现的遗传缺陷。
