Pacinda S J, Ledet S C, Gondo M M, Langston C, Brown R W, Carmona P A, Franklin R B, Roggli V L, Cagle P T
Department of Pathology, Baylor College of Medicine, Houston, TX, USA.
Hum Pathol. 1996 Jun;27(6):542-6. doi: 10.1016/s0046-8177(96)90159-8.
Pulmonary blastomas (PBs) are rare primary malignancies that include adult types: biphasic pulmonary blastoma (BPB) and well-differentiated fetal adenocarcinoma (WDFA); and childhood type: pleuropulmonary blastoma (PPB). Their pathogenesis and relationship to bronchogenic carcinoma (BCA) are controversial. To determine whether or not PB share molecular pathological features with BCA, the authors immunostained three BPB, three WDFA, three PPB, and 80 standard BCA for p53 protein and MDM2 protein, gene products believed to be significant in the pathogenesis of BCA. Paraffin-embedded tissue sections were immunostained with monoclonal antibody to p53 and MDM2 proteins. Strong intranuclear staining in greater than 10% of cells was considered positive. Three (50%) BPB and WDFA stained for p53 and five (83%) for MDM2. None of the PPB stained for p53, and one PPB did not stain for either p53 or MDM2. Five of six adult type PB occurred in smokers, whereas none of the PPB was associated with smoking. Seventy-five (94%) of the BCA stained for MDM2 and 46 (61%) for p53. Immunostaining patterns for p53 and MDM2 in adult types of PB, and not PPB, appear similar to those for BCA. This may suggest that adult type PB, but not childhood PB, have a similar pathogenesis to BCA.
肺母细胞瘤（PBs）是罕见的原发性恶性肿瘤，包括成人型：双相肺母细胞瘤（BPB）和高分化胎儿腺癌（WDFA）；以及儿童型：胸膜肺母细胞瘤（PPB）。它们的发病机制以及与支气管肺癌（BCA）的关系存在争议。为了确定PB是否与BCA具有共同的分子病理学特征，作者对3例BPB、3例WDFA、3例PPB以及80例标准BCA进行了p53蛋白和MDM2蛋白免疫染色，这两种基因产物被认为在BCA的发病机制中具有重要意义。用针对p53和MDM2蛋白的单克隆抗体对石蜡包埋的组织切片进行免疫染色。超过10%的细胞出现强核内染色被视为阳性。3例（50%）BPB和WDFA的p53染色呈阳性，5例（83%）的MDM2染色呈阳性。PPB中没有p53染色阳性的病例，1例PPB的p53和MDM2染色均为阴性。6例成人型PB中有5例发生在吸烟者中，而PPB均与吸烟无关。75例（94%）BCA的MDM2染色呈阳性，46例（61%）的p53染色呈阳性。成人型PB（而非PPB）中p53和MDM2的免疫染色模式与BCA相似。这可能表明成人型PB而非儿童型PB与BCA具有相似的发病机制。
