Boehme S A, Lenardo M J
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
J Immunol. 1996 Jun 1;156(11):4075-8.
The p53 protein plays an important role in various forms of thymocyte apoptosis, however its role in mature T lymphocyte death caused by TCR stimulation has not been examined. We demonstrate here that T cell blasts derived from mice containing a germ-line deficiency of the p53 tumor suppressor gene are susceptible to TCR-induced apoptosis to the same degree as wild-type T cells. TCR stimulation of both resting and proliferatingT cells results in up-regulated expression of the p53-induced genes Bax and p21, and the induction of these genes appears reduced in T cells that are deficient in p53. Thus, while activation of p53-dependent genes following TCR stimulation is defective in p53-/- T cells, there is no impairment in their ability to undergo apoptosis. These results suggest that TCR-mediated apoptosis of mature T cells takes place via a pathway that is independent of p53.
p53蛋白在各种形式的胸腺细胞凋亡中发挥重要作用，然而其在由TCR刺激引起的成熟T淋巴细胞死亡中的作用尚未得到研究。我们在此证明，源自p53肿瘤抑制基因种系缺陷小鼠的T细胞母细胞对TCR诱导的凋亡的敏感性与野生型T细胞相同。静止和增殖T细胞的TCR刺激均导致p53诱导基因Bax和p21的表达上调，而在p53缺陷的T细胞中这些基因的诱导似乎减少。因此，虽然TCR刺激后p53依赖基因的激活在p53 - / - T细胞中存在缺陷，但它们进行凋亡的能力没有受损。这些结果表明，成熟T细胞的TCR介导的凋亡通过独立于p53的途径发生。
