Svendsen Bollen L, Crowley A, Stodulski G, Hau J
Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, UK.
J Immunol Methods. 1996 May 27;191(2):113-20. doi: 10.1016/0022-1759(96)00010-5.
The antibody response in rabbits and chickens (serum and egg yolk) immunized with human immunoglobulin G was studied during a 1-year immunisation period comparing the efficacy of three adjuvants: Freund's complete adjuvant (FCA), Freund's incomplete adjuvant (FIA) and Hunter's TiterMax adjuvant (HTM). Monthly boosters of the antigen in saline were administered. Adjuvants were used in the initial immunisation and in a booster at week 26. FCA was found to result in the highest titre in both species. FIA was more efficient than HTM in mediating an antibody response in rabbits. In chickens, FIA resulted in similar antibody titres in serum and egg yolk. The effect of HTM was similar to FIA in chicken serum but lower in egg yolk. The titres obtained in rabbits were approximately a factor 1.5-2.0 higher than in the corresponding chicken groups. However, the antibody productivity of the chicken, when using the egg yolk as the antibody source, was a factor 5-10 higher than that of the rabbit depending on the adjuvant used. FCA and FIA resulted in similar antibody avidity in rabbits whereas HTM had a lesser effect. In chickens FCA initially resulted in the highest antibody avidity in both serum and egg yolk, but after the booster with adjuvant the antibody avidity became similar for all three adjuvants.
在为期1年的免疫期内，研究了用人免疫球蛋白G免疫的兔和鸡（血清和蛋黄）的抗体反应，比较了三种佐剂的效果：弗氏完全佐剂（FCA）、弗氏不完全佐剂（FIA）和亨特氏TiterMax佐剂（HTM）。每月用生理盐水给予抗原加强免疫。佐剂用于初次免疫和第26周的一次加强免疫。结果发现，FCA在两种动物中均产生最高滴度。在介导兔的抗体反应方面，FIA比HTM更有效。在鸡中，FIA在血清和蛋黄中产生相似的抗体滴度。HTM在鸡血清中的效果与FIA相似，但在蛋黄中的效果较低。兔中获得的滴度比相应鸡组高约1.5 - 2.0倍。然而，以蛋黄作为抗体来源时，鸡的抗体产生能力比兔高5 - 10倍，这取决于所使用的佐剂。FCA和FIA在兔中产生相似的抗体亲和力，而HTM的作用较小。在鸡中，FCA最初在血清和蛋黄中均产生最高的抗体亲和力，但在用佐剂加强免疫后，三种佐剂的抗体亲和力变得相似。
