Kux A, Bertram S, Hufert F T, Schmitz H, von Laer D
Abteilung Virologie, Universität Freiburg, Germany.
J Immunol Methods. 1996 May 27;191(2):179-86. doi: 10.1016/0022-1759(96)00020-8.
A flow cytometric assay (FCA), which detects the p24 antigen in HIV-infected cell lines and in peripheral blood mononuclear cells (PBMC) of AIDS patients, has been described in several studies. However, the results presented here clearly show that this p24-FCA, although useful for the analysis of HIV infection of cells in vitro, does not specifically detect HIV-infected PBMC from patients. Isotype control antibodies also stained PBMC from HIV-infected patients to a greater degree than the PBMC from healthy controls. Furthermore, the CD4-negative lymphocytes, which are generally not infected with HIV, were also found to stain with anti-p24. Finally, no enrichment of HIV-infected cells was found in the FACS-purified CD4+p24+ lymphocytes, compared to the CD4+p24- cell fraction. The p24-FCA, therefore, was not useful for determining the percentage of infected PBMCs from HIV-infected individuals.
多项研究中描述了一种流式细胞术检测法（FCA），该方法可检测HIV感染细胞系及艾滋病患者外周血单个核细胞（PBMC）中的p24抗原。然而，此处呈现的结果清楚表明，这种p24-FCA虽然对体外细胞的HIV感染分析有用，但并不能特异性检测出患者的HIV感染PBMC。同型对照抗体对HIV感染患者的PBMC染色程度也高于健康对照的PBMC。此外，通常未感染HIV的CD4阴性淋巴细胞也被发现可与抗p24发生染色反应。最后，与CD4+p24-细胞组分相比，在FACS纯化的CD4+p24+淋巴细胞中未发现HIV感染细胞的富集。因此，p24-FCA对于确定HIV感染个体中感染PBMC的百分比并无用处。
