Lewis P E, Warren J E
Prostaglandins. 1977 May;13(5):957-63. doi: 10.1016/0090-6980(77)90225-8.
Three groups of 6 ewes were laparotomized on day 9 of an estrous cycle (estrus = day 0) and the corporà lutea (CL) were marked with India ink. Indwelling cannulae were inserted into the uterine horn adjacent to the CL in groups 2 and 3. Group 1 was injected intramuscularly (i.m.) with corn oil twice daily on day 9. Group 2 received 750 microng 17beta-estradiol (E2) i.m. twice daily on day 9 plus intrauterine injections of indomethacin (INDO) vehicle on days 9 through 13. Group 3 received in the same estrogen treatment plus the injection of 20 mg INDO twice daily on days 9 through 13. Jugular venous samples were taken once daily on days 9 through 14 for progesterone analysis. At re-laparotmy on day 14, the ovaries were examined for new ovulations, and the ovary bearing the marked CL was removed. Results showed that E2 induced premature luteal regression as indicated by decreased CL weights and plasma progesterone levels. INDO when given in conjunction with E2 effectively blocked the luteolytic action of E2. These results suggest that the luteolytic action of E2 is mediated via increased prostaglandin secretion and release from the uterus.
在发情周期的第9天（发情期 = 第0天），将三组每组6只母羊进行剖腹手术，并用印度墨水标记黄体（CL）。在第2组和第3组中，将留置套管插入与CL相邻的子宫角。第1组在第9天每天两次肌肉注射（i.m.）玉米油。第2组在第9天每天两次肌肉注射750微克17β - 雌二醇（E2），并在第9天至第13天进行子宫内注射吲哚美辛（INDO）赋形剂。第3组接受相同的雌激素治疗，并在第9天至第13天每天两次注射20毫克INDO。在第9天至第14天每天采集一次颈静脉样本进行孕酮分析。在第14天再次剖腹手术时，检查卵巢是否有新的排卵，并切除带有标记CL的卵巢。结果表明，E2导致黄体过早退化，表现为CL重量和血浆孕酮水平降低。INDO与E2联合使用时可有效阻断E2的溶黄体作用。这些结果表明，E2的溶黄体作用是通过子宫中前列腺素分泌和释放的增加介导的。
