Hoyer G, Magness R, Huie M, Whysong G, Weems C
Prostaglandins Leukot Med. 1985 Feb;17(2):137-46. doi: 10.1016/0262-1746(85)90100-3.
Nonpregnant ewes were assigned as they came into estrus to one of the following randomized treatment groups: 1) Vehicle (1 ml corn oil) + Vehicle (buffer), 2) Estradiol-17 beta + Vehicle (buffer) or Estradiol-17 beta + PGE1 in buffer. Ewes were unilaterally ovariectomized on day 8 postestrus and an intrauterine cannula was installed in the uterine horn adjacent to the remaining luteal-bearing ovary. Buffer of PGE 1 (500 micrograms) in buffer was infused intrauterine every 4 hours from day 8 through day 15. Luteolysis was initiated by giving an intramuscular injection of estradiol-17 beta (500 micrograms) on days 9 and 10. Chronic intrauterine infusions of PGE1 maintained jugular progesterone through day 15 and weights of corpora lutea and progesterone in corpora lutea on day 15 although luteolysis was initiated by estradiol. It is concluded that chronic intrauterine infusions of PGE1 can prevent an estrogen-induced premature luteolysis.
1）赋形剂（1毫升玉米油）+赋形剂（缓冲液），2）雌二醇-17β+赋形剂（缓冲液）或缓冲液中的雌二醇-17β+前列腺素E1。母羊在发情期后第8天进行单侧卵巢切除术，并在与保留有黄体的卵巢相邻的子宫角安装宫内插管。从第8天到第15天，每4小时向子宫内注入500微克前列腺素E1缓冲液。在第9天和第10天通过肌肉注射500微克雌二醇-17β启动黄体溶解。尽管黄体溶解由雌二醇启动，但慢性子宫内注入前列腺素E1可使颈静脉孕酮水平维持到第15天，并使第15天黄体的重量和黄体中的孕酮水平保持稳定。结论是，慢性子宫内注入前列腺素E1可预防雌激素诱导的过早黄体溶解。
