Bergeron D, Audette M, Lambert R D
Department of Obstetrics, Laval University, Ste-Foy, Québec, Canada.
J Reprod Fertil. 1996 Jan;106(1):143-51. doi: 10.1530/jrf.0.1060143.
The mechanisms underlying the inhibition of lymphocyte proliferative response by rabbit blastocoelic fluid collected on day 12 of embryonic development were investigated. Treatment with blastocoelic fluid, even in the presence of concanavalin A, maintains lymphocytes in a quiescent state by preventing cell entry into the S phase of the cell cycle. Gene expression of interleukin 2 receptor is completely blocked by treatment with blastocoelic fluid as are the secretion and gene expression of interleukin 2. Addition of interleukin 2 to prestimulated interleukin 2 receptor positive lymphocytes failed to downregulate the expression of high-affinity interleukin 2 receptor and completely abolished the embryonic fluid-mediated inhibitory effect on [3H]thymidine incorporation. Taken together, these results suggest that embryonic fluid has differential inhibitory effects, depending on the activation state of the lymphocytes. Nevertheless, inhibition of interleukin 2 and interleukin 2 receptor expression by embryonic fluid restrains immune cell activity and therefore can be implicated in the survival of the fetal semi-allograft.
对胚胎发育第12天收集的兔囊胚液抑制淋巴细胞增殖反应的潜在机制进行了研究。用囊胚液处理，即使在存在伴刀豆球蛋白A的情况下，也通过阻止细胞进入细胞周期的S期，使淋巴细胞维持在静止状态。囊胚液处理可完全阻断白细胞介素2受体的基因表达，以及白细胞介素2的分泌和基因表达。将白细胞介素2添加到预刺激的白细胞介素2受体阳性淋巴细胞中，未能下调高亲和力白细胞介素2受体的表达，并且完全消除了胚胎液介导的对[3H]胸腺嘧啶核苷掺入的抑制作用。综上所述，这些结果表明，胚胎液具有不同的抑制作用，这取决于淋巴细胞的激活状态。然而，胚胎液对白细胞介素2和白细胞介素2受体表达的抑制作用会抑制免疫细胞活性，因此可能与胎儿半同种异体移植物的存活有关。
