Lambert R D, Gosselin S, Pothier F, Morin C, Veilleux R, Roy M, Fortier M A
Unité de Recherches en Ontogénie et Reproduction, Le Centre Hospitalier de l'Université Laval, Quebec, Canada.
Int J Cancer. 1989 Sep 15;44(3):524-8. doi: 10.1002/ijc.2910440325.
To determine whether rabbit blastocoelic fluid could inhibit tumor-cell proliferation, day-9 and day-12 embryonic fluids, together with autologous and homologous sera, were collected from pregnant or pseudopregnant rabbits and tested against 13 different cell lines and on human carcinoma cells in primary culture. An inhibitory effect on cell proliferation was observed in the presence of blastocoelic fluids, but not with homologous or heterologous sera. This suppression was higher with samples collected at day 12 than at day 9 of pregnancy. No such inhibition could be detected on one-cell rabbit embryos or on freshly prepared uterine stromal or myometrial cells. In addition, the inhibitory activity on tumor cells was completely reversible upon removal of the fluids. Incorporation of 3H-thymidine, 3H-uridine and 35S-methionine revealed that, in the presence of blastocoelic fluids, both DNA and RNA syntheses were rapidly inhibited. Inhibition of protein synthesis did not occur before 24 hr of treatment. We conclude that rabbit blastocoelic fluid suppresses the proliferation of tumor cells via inhibition of RNA and DNA synthesis by a process which may involve the expression of growth-suppression gene(s).
为了确定兔囊胚液是否能抑制肿瘤细胞增殖，从怀孕或假孕兔收集第9天和第12天的胚胎液，以及自体和同源血清，并针对13种不同细胞系和原代培养的人癌细胞进行检测。在囊胚液存在的情况下观察到对细胞增殖有抑制作用，但同源或异源血清则无此作用。怀孕第12天收集的样本比第9天收集的样本抑制作用更强。在单细胞兔胚胎或新鲜制备的子宫基质或肌层细胞上未检测到这种抑制作用。此外，去除囊胚液后，对肿瘤细胞的抑制活性完全可逆。掺入³H-胸腺嘧啶核苷、³H-尿苷和³⁵S-甲硫氨酸显示，在囊胚液存在的情况下，DNA和RNA合成均迅速受到抑制。蛋白质合成在处理24小时前未受到抑制。我们得出结论，兔囊胚液通过抑制RNA和DNA合成来抑制肿瘤细胞增殖，这一过程可能涉及生长抑制基因的表达。
