Hunt G J, Tabachnick W J
Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071, USA.
J Med Entomol. 1996 May;33(3):271-7. doi: 10.1093/jmedent/33.3.271.
Equipment and procedures are described for biosafety level 3 (BL-3) containment work with small, zoophilic arthropods. BL-3 classified pathogens always must be manipulated in biological safety cabinets. Procedures, including physical barriers and handling methods, that prevent the escape of potentially virus-infected insects are discussed, and the use of a monitoring system for insect security is explained. The inability to recover escaped minute, flying insects poses a major difference from similar work with larger insects, such as mosquitoes. Methods were developed for the safe and secure handling of Culicoides variipennis sonorensis Wirth & Jones infected with exotic bluetongue viruses during BL-3 containment.
本文描述了用于对小型嗜动物性节肢动物进行生物安全3级（BL - 3）防护工作的设备和程序。BL - 3分类病原体必须始终在生物安全柜中进行操作。讨论了包括物理屏障和处理方法在内的防止潜在病毒感染昆虫逃逸的程序，并解释了昆虫安全监测系统的使用。无法找回逃逸的微小飞行昆虫是与处理较大昆虫（如蚊子）的类似工作的主要区别。开发了在BL - 3防护条件下安全处理感染外来蓝舌病病毒的索诺拉库蠓的方法。
