一种用于秀丽隐杆线虫整体胚胎原位杂交的多孔板版本。
A multi-well version of in situ hybridization on whole mount embryos of Caenorhabditis elegans.
作者信息
Tabara H, Motohashi T, Kohara Y
机构信息
Department of Genetics, The Graduate University of Advanced Studies, Japan.
出版信息
Nucleic Acids Res. 1996 Jun 1;24(11):2119-24. doi: 10.1093/nar/24.11.2119.
Abstract
We report an efficient procedure for in situ hybridization with a multi-well format on Caenorhabditis elegans embryos for large scale screening of gene expression patterns in this organism. Each hybridization well contains embryos at various stages throughout embryogenesis. The validity of the method was confirmed through results with control genes whose expression patterns have been reported; glp-1 in very early embryos, myo-2 in pharyngeal muscle and unc-54 in body wall muscle. Several collagen genes and a pepsinogen gene were also examined to establish a set of lineage-specific markers. As a pilot project, we examined approximately 100 unique cDNA species classified by our cDNA project, finding that approximately 10% of the cDNA groups were expressed in specific cells and at specific stages.
摘要
我们报道了一种在秀丽隐杆线虫胚胎上进行多孔原位杂交的高效方法，用于大规模筛选该生物体中的基因表达模式。每个杂交孔都包含处于胚胎发育各个阶段的胚胎。通过使用已报道其表达模式的对照基因的结果，证实了该方法的有效性；在极早期胚胎中的glp-1、咽肌中的myo-2和体壁肌中的unc-54。还检查了几个胶原蛋白基因和一个胃蛋白酶原基因，以建立一组谱系特异性标记。作为一个试点项目，我们检查了约100种由我们的cDNA项目分类的独特cDNA物种，发现约10%的cDNA组在特定细胞和特定阶段表达。
相似文献
1
A multi-well version of in situ hybridization on whole mount embryos of Caenorhabditis elegans.一种用于秀丽隐杆线虫整体胚胎原位杂交的多孔板版本。
Nucleic Acids Res. 1996 Jun 1;24(11):2119-24. doi: 10.1093/nar/24.11.2119.
2
Autonomy and nonautonomy in cell fate specification of muscle in the Caenorhabditis elegans embryo: a reciprocal induction.秀丽隐杆线虫胚胎中肌肉细胞命运特化的自主性与非自主性：一种相互诱导作用
Science. 1994 Mar 11;263(5152):1449-52. doi: 10.1126/science.8128230.
3
lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. elegans.lag-2可能编码一种秀丽隐杆线虫GLP-1和LIN-12受体的信号配体。
Development. 1994 Oct;120(10):2913-24. doi: 10.1242/dev.120.10.2913.
4
A novel C-terminal kinesin subfamily may be involved in chromosomal movement in caenorhabditis elegans.一个新的C端驱动蛋白亚家族可能参与秀丽隐杆线虫的染色体运动。
FEBS Lett. 2000 Mar 17;470(1):70-6. doi: 10.1016/s0014-5793(00)01258-8.
5
Translational control of maternal glp-1 mRNA establishes an asymmetry in the C. elegans embryo.母体glp-1 mRNA的翻译控制在秀丽隐杆线虫胚胎中建立了一种不对称性。
Cell. 1994 Apr 22;77(2):183-94. doi: 10.1016/0092-8674(94)90311-5.
6
Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle.秀丽隐杆线虫的双功能乙醛酸循环蛋白：一种在发育过程中受调控的肠和肌肉蛋白。
Dev Biol. 1995 Jun;169(2):399-414. doi: 10.1006/dbio.1995.1156.
7
cDNA cloning and expression of a C-terminus motor kinesin-like protein KLP-17, involved in chromosomal movement in Caenorhabditis elegans.参与秀丽隐杆线虫染色体运动的C端马达驱动蛋白样蛋白KLP-17的cDNA克隆与表达
Biochem Biophys Res Commun. 2000 Jan 19;267(2):643-50. doi: 10.1006/bbrc.1999.1866.
8
MEC-8 regulates alternative splicing of unc-52 transcripts in C. elegans hypodermal cells.MEC-8调控秀丽隐杆线虫皮下细胞中unc-52转录本的可变剪接。
Development. 2002 Nov;129(21):4999-5008. doi: 10.1242/dev.129.21.4999.
9
APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos.APH-1是一种多次跨膜蛋白，对秀丽隐杆线虫胚胎中的Notch信号通路至关重要。
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):775-9. doi: 10.1073/pnas.022523499. Epub 2002 Jan 15.
10
Genetic redundancy in endoderm specification within the genus Caenorhabditis.秀丽隐杆线虫属内胚层特化中的遗传冗余。
Dev Biol. 2005 Aug 15;284(2):509-22. doi: 10.1016/j.ydbio.2005.05.016.
引用本文的文献
1
A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes.一个小 RNA 系统确保了减数分裂染色体的同源配对和未配对沉默的准确性。
EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. Epub 2023 Apr 20.
2
Multiview confocal super-resolution microscopy.多视场共聚焦超分辨显微镜。
Nature. 2021 Dec;600(7888):279-284. doi: 10.1038/s41586-021-04110-0. Epub 2021 Nov 26.
3
A new anterior pharyngeal region specific fluorescent co-transformation marker.一种新的咽前区特异性荧光共转化标记物。
MicroPubl Biol. 2019 Jan 16;2019. doi: 10.17912/micropub.biology.000084.
4
Electropermeabilization-based fluorescence hybridization of whole-mount plant parasitic nematode specimens.基于电通透法的全株植物寄生线虫标本荧光杂交
MethodsX. 2019 Nov 13;6:2720-2728. doi: 10.1016/j.mex.2019.11.009. eCollection 2019.
5
Long non-coding RNA Irm enhances myogenic differentiation by interacting with MEF2D.长非编码 RNA Irm 通过与 MEF2D 相互作用增强成肌分化。
Cell Death Dis. 2019 Feb 21;10(3):181. doi: 10.1038/s41419-019-1399-2.
6
OpenHiCAMM: High-Content Screening Software for Complex Microscope Imaging Workflows.OpenHiCAMM：用于复杂显微镜成像工作流程的高内涵筛选软件。
iScience. 2018 Apr 27;2:136-140. doi: 10.1016/j.isci.2018.03.017.
7
Microinjection.显微注射
Bio Protoc. 2017 Oct 5;7(19). doi: 10.21769/BioProtoc.2565.
8
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans.使用肽核酸（PNA）探针通过荧光原位杂交（FISH）技术对线虫端粒和重复序列进行观察与定量分析
J Vis Exp. 2016 Aug 4(114):54224. doi: 10.3791/54224.
9
A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans.一种用于检测秀丽隐杆线虫中微小RNA的全组织原位杂交方法。
RNA. 2016 Jul;22(7):1099-106. doi: 10.1261/rna.054239.115. Epub 2016 May 6.
10
Asymmetric transcript discovery by RNA-seq in C. elegans blastomeres identifies neg-1, a gene important for anterior morphogenesis.通过RNA测序在秀丽隐杆线虫卵裂球中发现不对称转录本，鉴定出neg-1，这是一个对前部形态发生很重要的基因。
PLoS Genet. 2015 Apr 13;11(4):e1005117. doi: 10.1371/journal.pgen.1005117. eCollection 2015 Apr.
本文引用的文献
1
Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank.利用冷冻转座子插入突变体库在秀丽隐杆线虫中进行靶向基因失活。
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7431-5. doi: 10.1073/pnas.90.16.7431.
2
Translational control of maternal glp-1 mRNA establishes an asymmetry in the C. elegans embryo.母体glp-1 mRNA的翻译控制在秀丽隐杆线虫胚胎中建立了一种不对称性。
Cell. 1994 Apr 22;77(2):183-94. doi: 10.1016/0092-8674(94)90311-5.
3
Fluorescence in situ hybridisation of multiple probes on a single microscope slide.在一张显微镜载玻片上对多个探针进行荧光原位杂交。
Nucleic Acids Res. 1994 Sep 11;22(18):3689-92. doi: 10.1093/nar/22.18.3689.
4
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.来自秀丽隐杆线虫三号染色体的2.2兆碱基连续核苷酸序列。
Nature. 1994 Mar 3;368(6466):32-8. doi: 10.1038/368032a0.
5
Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans.秀丽隐杆线虫胚胎RNA分布中的体细胞-种系不对称性。
Development. 1994 Oct;120(10):2823-34. doi: 10.1242/dev.120.10.2823.
6
Expression patterns of predicted genes from the C. elegans genome sequence visualized by FISH in whole organisms.通过全生物体荧光原位杂交（FISH）可视化秀丽隐杆线虫基因组序列中预测基因的表达模式。
Nat Genet. 1995 Nov;11(3):314-20. doi: 10.1038/ng1195-314.
7
Developmental expression pattern screen for genes predicted in the C. elegans genome sequencing project.针对秀丽隐杆线虫基因组测序计划中预测的基因进行发育表达模式筛选。
Nat Genet. 1995 Nov;11(3):309-13. doi: 10.1038/ng1195-309.
8
The embryonic cell lineage of the nematode Caenorhabditis elegans.秀丽隐杆线虫的胚胎细胞谱系。
Dev Biol. 1983 Nov;100(1):64-119. doi: 10.1016/0012-1606(83)90201-4.
9
Number and organization of collagen genes in Caenorhabditis elegans.秀丽隐杆线虫中胶原蛋白基因的数量与组织方式。
Mol Cell Biol. 1984 Nov;4(11):2389-95. doi: 10.1128/mcb.4.11.2389-2395.1984.
10
Immunochemical localization of myosin heavy chain isoforms and paramyosin in developmentally and structurally diverse muscle cell types of the nematode Caenorhabditis elegans.肌球蛋白重链亚型和副肌球蛋白在秀丽隐杆线虫发育和结构多样的肌肉细胞类型中的免疫化学定位。
J Cell Biol. 1987 Dec;105(6 Pt 1):2763-70. doi: 10.1083/jcb.105.6.2763.
Zotero 插件