Fontana J D, Guimarães M F, Martins N T, Fontana C A, Baron M
Department of Biochemistry, Federal University of Paraná (LQBB-UFPR), Curitiba-PR-Brazil.
Appl Biochem Biotechnol. 1996 Spring;57-58:413-22. doi: 10.1007/BF02941721.
Growth of the yeast Phaffia rhodozyma was carried out in a simplified medium based on less expensive nutrient sources, such as diluted sugar cane juice, urea, and sodium phosphate. The usual content of the astaxanthin, an oxygenated pink carotenoid useful for fish flesh staining, was improved along with with good cell yields (respective values of > 1300 micrograms/g cells and > 5 g cells/L were observed). Yeast invertase and urease must therefore play an important role in the implementation of low-cost culture media.
利用稀释甘蔗汁、尿素和磷酸钠等成本较低的营养源，在一种简化培养基中培养红法夫酵母。虾青素是一种用于鱼肉染色的氧化型粉红色类胡萝卜素，其通常含量有所提高，同时细胞产量也很高（分别观察到>1300微克/克细胞和>5克细胞/升的值）。因此，酵母转化酶和脲酶在低成本培养基的应用中必定发挥着重要作用。
