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本文引用的文献

1
Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples.用于生物样本中肠道病毒RNA高灵敏度检测的巢式聚合酶链反应
J Clin Microbiol. 1993 May;31(5):1345-9. doi: 10.1128/jcm.31.5.1345-1349.1993.
2
Detection of enterovirus RNA in clinical samples by nested polymerase chain reaction for rapid diagnosis of enterovirus infection.通过巢式聚合酶链反应检测临床样本中的肠道病毒RNA以快速诊断肠道病毒感染。
J Virol Methods. 1994 Jul;48(2-3):155-66. doi: 10.1016/0166-0934(94)90115-5.
3
Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization.利用酶促扩增和核酸杂交技术对肠道病毒进行分子检测与鉴定。
J Clin Microbiol. 1990 May;28(5):843-50. doi: 10.1128/jcm.28.5.843-850.1990.
4
Enzymatic RNA amplification of the enteroviruses.肠道病毒的酶促RNA扩增
J Clin Microbiol. 1990 Mar;28(3):438-42. doi: 10.1128/jcm.28.3.438-442.1990.

通过逆转录聚合酶链反应诊断的肠道病毒性咽炎。

Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction.

作者信息

Sharland M, Hodgson J, Davies E G, Booth J, Jeffery S

机构信息

Paediatric Infectious Diseases Unit, St George's Hospital, London.

出版信息

Arch Dis Child. 1996 May;74(5):462-3. doi: 10.1136/adc.74.5.462.

DOI:10.1136/adc.74.5.462
PMID:8669969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1511539/
Abstract

The role of enteroviruses in childhood pharyngitis was investigated using enteroviral specific reverse transcriptase-polymerase chain reaction (RT-PCR). Viral/bacterial throat swabs were taken from 50 children with acute pharyngitis and 26 controls. A positive culture was identified in only 26% of children with pharyngitis (adenovirus 10%, group A streptococci 2%), and none of the controls. Enteroviral RT-PCR was positive in 8% of the pharyngitis group and none of the controls. Enteroviruses are an important cause of pharyngitis in childhood.

摘要

采用肠道病毒特异性逆转录聚合酶链反应(RT-PCR)研究肠道病毒在儿童咽炎中的作用。从50例急性咽炎患儿和26例对照者中采集咽喉部病毒/细菌拭子。仅26%的咽炎患儿培养结果呈阳性(腺病毒10%,A组链球菌2%),而对照者均为阴性。8%的咽炎组患儿肠道病毒RT-PCR结果呈阳性,对照者均为阴性。肠道病毒是儿童咽炎的一个重要病因。