Vallejo C G, López M, Ochoa P, Manzanares M, Garesse R
Instituto de Investigaciones Biomédicas (CSIC) and Departamento de Bioquímica, Facultad de Medicina (UAM), Madrid, Spain.
Biochem J. 1996 Mar 1;314 ( Pt 2)(Pt 2):505-10. doi: 10.1042/bj3140505.
During the early development of Artemia there is an increase in mitochondrial enzyme activities of about one order of magnitude, whereas the activities of two cytoplasmic enzymes tested as controls remain unaltered. The mitochondrial enzyme activation correlates with (i) large changes in mitochondrial morphology, (ii) a 5-fold increase in the amount of the H+-ATP synthase beta-subunit and (iii) a dramatic increase in the steady-state level of mitochondrial mRNAs, whereas mitochondrial rRNA concentrations remain mostly unchanged. In contrast, the level of mitochondrial DNA does not change significantly during the first 20 h after resumption of development. After hatching, the mitochondrial DNA content increases twice in parallel with one round of cellular division, thus indicating that mitochondrial and nuclear replication are coupled in Artemia postgastrular development. The data presented strongly suggest that mitochondrial maturation in the absence of significant mitochondrial proliferation is responsible for the dramatic increase in mitochondrial function that takes place after resumption of development in Artemia.
在卤虫早期发育过程中，线粒体酶活性增加了约一个数量级，而作为对照测试的两种细胞质酶的活性保持不变。线粒体酶的激活与以下因素相关：（i）线粒体形态的巨大变化；（ii）H⁺-ATP合酶β亚基数量增加5倍；（iii）线粒体mRNA稳态水平急剧增加，而线粒体rRNA浓度大多保持不变。相比之下，在发育恢复后的前20小时内，线粒体DNA水平没有显著变化。孵化后，线粒体DNA含量随着一轮细胞分裂平行增加两倍，这表明在卤虫原肠后期发育中线粒体和核复制是耦合的。所呈现的数据强烈表明，在没有显著线粒体增殖的情况下线粒体成熟是卤虫发育恢复后线粒体功能急剧增加的原因。
