Peña P, Garesse R
Departamento de Bioqumica (UAM), Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):785-91. doi: 10.1006/bbrc.1993.2114.
The cDNA encoding the Drosophila melanogaster beta subunit of H+ ATP synthase has been cloned and sequenced. The predicted mature protein is highly homologous to the equivalent beta subunits of other organisms and is preceded by a signal peptide of 31 amino acids, that although not conserved at primary sequence level has the characteristics of leader peptides present in other mitochondrial proteins. We have raised polyclonal antibodies that specifically recognize the beta H+ ATP synthase subunit present in Drosophila melanogaster protein extracts. This is the first time that a gene of the ATP synthase complex has been characterized in the invertebrate phyla.
编码果蝇H⁺-ATP合酶β亚基的cDNA已被克隆和测序。预测的成熟蛋白质与其他生物的等效β亚基高度同源，并且前面有一个31个氨基酸的信号肽，尽管该信号肽在一级序列水平上不保守，但具有其他线粒体蛋白质中存在的前导肽的特征。我们制备了多克隆抗体，该抗体能特异性识别果蝇蛋白质提取物中存在的β-H⁺-ATP合酶亚基。这是首次在无脊椎动物门中对ATP合酶复合体的一个基因进行表征。
