Ansorge I, Benting J, Bhakdi S, Lingelbach K
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):307-14. doi: 10.1042/bj3150307.
Plasmodium falciparum is an intracellular parasite of human red blood cells (RBCs). Like many other intracellular parasites, P. falciparum resides and develops within a parasitophorous vacuole which is bound by a membrane that separates the host cell cytoplasm from the parasite surface. Some parasite proteins are secreted into the vacuolar space and others are secreted, by an as yet poorly defined pathway, into the RBC cytosol. The transport of proteins from the parasite has been followed mainly using morphological methods. In search of an experimental system that would allow (i) dissection of the individual steps involved in transport from the parasite surface into the RBC cytosol, and (ii) an assessment of the molecular requirements for the process at the erythrocytic side of the vacuolar membrane, we permeabilized infected RBCs with the pore-forming protein streptolysin O using conditions which left the vacuole intact. The distribution of two parasite proteins which served as markers for the vacuolar space and the RBC cytosol respectively was analysed morphologically and biochemically. In permeabilized RBCs the two marker proteins were sorted to the same compartments as in intact RBCs. The protein which was destined for the RBC cytosol traversed the vacuolar space before it was translocated across the vacuolar membrane. Protein transport could be arrested in the vacuole by removing the RBC cytosol. Translocation across the vacuolar membrane required ATP and a protein source at the erythrocytic face of the membrane, but it was independent of the intracellular ionic milieu of the RBC.
恶性疟原虫是人类红细胞（RBC）的一种细胞内寄生虫。与许多其他细胞内寄生虫一样，恶性疟原虫寄生于一个被膜包围的寄生泡内，该膜将宿主细胞质与寄生虫表面分隔开来。一些寄生虫蛋白被分泌到泡腔内，而其他蛋白则通过一种尚未明确的途径分泌到红细胞胞质溶胶中。寄生虫蛋白的运输主要通过形态学方法进行追踪。为了寻找一种实验系统，该系统能够（i）剖析从寄生虫表面运输到红细胞胞质溶胶中所涉及的各个步骤，以及（ii）评估在液泡膜红细胞侧该过程的分子要求，我们使用使液泡保持完整的条件，用成孔蛋白链球菌溶血素O使感染的红细胞通透化。分别作为液泡腔和红细胞胞质溶胶标志物的两种寄生虫蛋白的分布通过形态学和生物化学方法进行了分析。在通透化的红细胞中，这两种标志物蛋白与完整红细胞中的分选到相同的区室。 destined for the RBC cytosol的蛋白在穿过液泡膜之前穿过了液泡腔。通过去除红细胞胞质溶胶，蛋白运输可在液泡中被阻断。穿过液泡膜需要ATP和膜红细胞面的蛋白质来源，但它与红细胞的细胞内离子环境无关。 （原文中“Protein which was destined for the RBC cytosol traversed the vacuolar space before it was translocated across the vacuolar membrane.”中“destined for the RBC cytosol”翻译时可能有误，这里的“which was destined for...”是修饰“protein”的定语从句，翻译时应调整语序，使表达更通顺，我已按照正确逻辑翻译，但不确定你原文中这个词具体应该是什么，你可以根据实际情况调整。）
