Redman K L, Burris G W
Indiana University School of Medicine, Fort Wayne Center for Medical Education, Fort Wayne, IN 46805, U.S.A.
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):315-21. doi: 10.1042/bj3150315.
Rat cDNAs for a 52-amino-acid ribosomal protein (CEP52) that is typically formed as a ubiquitin fusion protein, were cloned following reverse transcription and PCR amplification. CEP52 sequence conservation is demonstrated by the similarity of the human and rat cDNA sequences and the identity of the predicted proteins. Amplification of rat cDNA with a primer specific for the 3' non-coding region of the CEP52 gene, in combination with a consensus primer for the 5' end of the ubiquitin coding sequence, provided evidence that the rat CEP52 gene is fused to a ubiquitin reading frame. Direct sequence analysis of this PCR product confirmed the in-frame fusion of a ubiquitin coding sequence to the rat CEP52 gene. Antibodies against a synthetic CEP52 peptide were used to show that expressed CEP52 is associated with the 60 S ribosomal subunit, and that is is not linked to ubiquitin. The quantity of CEP52 found in different tissues is quite variable, but appears to correspond to the amount of ribosomes present. Although the human, Arabidopsis thaliana and Nicotiana tabacum CEP52 genes contain introns within the CEP52 coding region, the rat CEP52 coding sequence appears to lack insertions.
一种通常作为泛素融合蛋白形成的含52个氨基酸的核糖体蛋白（CEP52）的大鼠cDNA，经逆转录和PCR扩增后被克隆。人和大鼠cDNA序列的相似性以及预测蛋白质的同一性证明了CEP52序列的保守性。用针对CEP52基因3'非编码区的特异性引物与泛素编码序列5'端的共有引物扩增大鼠cDNA，提供了大鼠CEP52基因与泛素阅读框融合的证据。对该PCR产物的直接序列分析证实了泛素编码序列与大鼠CEP52基因的读框内融合。针对合成的CEP52肽的抗体用于表明表达的CEP52与60S核糖体亚基相关，并且它不与泛素相连。在不同组织中发现的CEP52的量变化很大，但似乎与存在的核糖体数量相对应。尽管人、拟南芥和烟草的CEP52基因在CEP52编码区内含有内含子，但大鼠CEP52编码序列似乎没有插入序列。
