非洲爪蟾TFIIS的克隆及其在卵母细胞和早期胚胎中的表达。

Cloning of Xenopus TFIIS and its expression in oocytes and early embryos.

作者信息

Kugawa F, Shinga J, Imagawa M, Arae K, Nagano M, Shibata M, Shiokawa K, Aoki M

机构信息

Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon University, Chiba, Japan.

出版信息

Biochem Biophys Res Commun. 1996 May 15;222(2):541-6. doi: 10.1006/bbrc.1996.0780.

DOI:10.1006/bbrc.1996.0780

PMID:8670241

Abstract

The transcriptional factor TFIIS has been cloned from Xenopus laevis. The length of the cDNA is 1668bp and contains the complete open reading frame of 303 amino acids. Xenopus TFIIS has high homologies to its human and mouse counterparts. In Northern blot analyses, TFIIS mRNAs that consisted of four different sizes were expressed relatively highly from the early stages of Xenopus oogenesis. During oocyte maturation, the pattern of Xenopus TFIIS messages showed a transient peak of expression. TFIIS mRNA occurred maternally and its level increased in later stage embryos. These data suggest that TFIIS mRNA is expressed in a developmentally regulated way in Xenopus laevis.

摘要

转录因子TFIIS已从非洲爪蟾中克隆出来。cDNA长度为1668bp，包含303个氨基酸的完整开放阅读框。非洲爪蟾TFIIS与其人类和小鼠对应物具有高度同源性。在Northern印迹分析中，由四种不同大小组成的TFIIS mRNA在非洲爪蟾卵子发生早期表达相对较高。在卵母细胞成熟过程中，非洲爪蟾TFIIS信息的模式显示出一个短暂的表达峰值。TFIIS mRNA由母体产生，其水平在后期胚胎中增加。这些数据表明，TFIIS mRNA在非洲爪蟾中以发育调控的方式表达。