Yasunami M, Suzuki K, Ohkubo H
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan.
Biochem Biophys Res Commun. 1996 Nov 12;228(2):365-70. doi: 10.1006/bbrc.1996.1667.
We cloned two novel types of TEA domain-containing transcription factor (ETFR-1 and -2) cDNAs. Amino acid sequences deduced for ETFR-1 and -2 as well as those of other known proteins of the same family, TEF-1 and ETF, exhibited significant identity (63-75% overall) to each other not only in the TEA DNA binding domain but also in the C-terminal regions. Northern blot analysis revealed that both the mRNAs were expressed in embryos as well as in many adult tissues, although their levels of expression varied. The results demonstrate that the mammalian TEA domain-containing transcription factor family consists of at least four distinct members, TEF-1, ETF, ETFR-1, and ETFR-2, which exhibit overlapping but differing spatiotemporal expression patterns, suggesting their redundant yet unique roles involved in not only developmental control but also tissue-specific regulation.
我们克隆了两种新型含TEA结构域的转录因子（ETFR-1和-2）的cDNA。推导得出的ETFR-1和-2的氨基酸序列，以及同一家族其他已知蛋白TEF-1和ETF的氨基酸序列，不仅在TEA DNA结合结构域，而且在C端区域都显示出彼此之间显著的一致性（总体为63-75%）。Northern印迹分析表明，尽管它们的表达水平有所不同，但这两种mRNA在胚胎以及许多成体组织中均有表达。结果表明，哺乳动物含TEA结构域的转录因子家族至少由四个不同成员组成，即TEF-1、ETF、ETFR-1和ETFR-2，它们表现出重叠但不同的时空表达模式，这表明它们不仅在发育控制中，而且在组织特异性调节中发挥着冗余但独特的作用。
