Taki T, Ohnishi T, Yamamoto A, Hiraga S, Arita N, Izumoto S, Hayakawa T, Morita T
Department of Neurosurgery, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):434-8. doi: 10.1006/bbrc.1996.0911.
The mammalian RAD51 gene is a homologue of the yeast RAD51 and E. coli RecA genes, which are involved in recombination and DNA repair. We examined the role of RAD51 protein in mouse cells using RAD51 antisense phosphorothioate oligonucleotides (ODNs). The extraluminal delivery of 50 nM or 100 nM of antisense ODNs with lipofection to mouse cells resulted 90% suppression of RAD51 protein expression. The antisense ODNs significantly inhibited the cell growth and the treated cells became more sensitive to gamma-irradiation than the control groups. These results indicate mouse RAD51 plays an essential role in cell proliferation and radioresistant activity.
哺乳动物的RAD51基因是酵母RAD51基因和大肠杆菌RecA基因的同源物，它们参与重组和DNA修复。我们使用RAD51反义硫代磷酸酯寡核苷酸（ODN）研究了RAD51蛋白在小鼠细胞中的作用。通过脂质转染将50 nM或100 nM反义ODN胞外递送至小鼠细胞，导致RAD51蛋白表达受到90%的抑制。反义ODN显著抑制细胞生长，且处理后的细胞对γ射线照射比对照组更敏感。这些结果表明小鼠RAD51在细胞增殖和抗辐射活性中起重要作用。
