Costagliola C, Balestrieri P, Fioretti F, Frunzio S, Rinaldi M, Scibelli G
Eye Clinic, II University of Naples, Italy.
Curr Eye Res. 1996 Apr;15(4):355-61. doi: 10.3109/02713689608995825.
Twenty male albino rabbits were studied. Four animals served as controls; the remaining 16 animals represented the treated group. All the treated animals were exposed to the same amount of energy delivered by the excimer laser (pulse rate: 20 Hz, fluence 250mJ/cm2; number of pulses: 6032; cumulative UV dose 1508 J/cm2) and were divided into eight groups of 2 animals each (four eyes). Samples of aqueous humor and lens were obtained at the following intervals: 5, 10, 20 and 40 min and 1, 2, 3 and 4 weeks after photorefractive keratectomy (PRK). The levels of reduced and oxidized glutathione, hydrogen peroxide, ascorbic acid and malondialdehyde were determined. Aqueous humor analyses, twenty min after PRK, showed no significant differences with pre-treatment values, while the observed variations in lens were constantly present over the entire follow-up period (one month). These findings suggest that the biochemical lens alterations induced by PRK may represent the earliest events relevant to cataractogenesis in the rabbit.
对20只雄性白化兔进行了研究。4只动物作为对照；其余16只动物代表治疗组。所有治疗组动物均接受准分子激光传递的相同能量（脉冲频率：20Hz，能量密度250mJ/cm²；脉冲数：6032；累积紫外线剂量1508J/cm²），并分为8组，每组2只动物（四只眼）。在以下时间间隔采集房水和晶状体样本：屈光性角膜切削术（PRK）后5、10、20和40分钟以及1、2、3和4周。测定了还原型和氧化型谷胱甘肽、过氧化氢、抗坏血酸和丙二醛的水平。PRK后20分钟的房水分析显示与治疗前值无显著差异，而在整个随访期（一个月）内，晶状体中观察到的变化持续存在。这些发现表明，PRK诱导的晶状体生化改变可能代表了兔白内障形成相关的最早事件。
