Nataraj C, Brown M L, Poston R M, Shawar S M, Rich R R, Lindahl K F, Kurlander R J
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
Int Immunol. 1996 Mar;8(3):367-78. doi: 10.1093/intimm/8.3.367.
Mice infected with Listeria monocytogenes (LM) generate H2-M3wt-restricted CD8 effectors which recognize a heat-killed LM-associated antigen (HAA) presented by macrophages. To characterize HAA, we extracted a bioactive component from LM using SDS or NaOH. Extracted HAA aggregated in hydrophilic solvents but dissociated in the presence of SDS into a smaller subunit which migrated in Sephadex G-200 between chymotrypsinogen (25 kDa) and cytochrome c (12.5 kDa). HAA bioactivity and size was unaffected by proteinase K under conditions which degraded virtually all detectable protein. HAA was also unaffected by other proteases, RNase and DNase, but HAA bioactivity was destroyed by periodate, an agent that degrades carbohydrates. These studies demonstrate that H2-M3wt can present a hydrophobic, non-peptide, microbial antigen, probably glycolipid in origin, to CD8 T cells.
感染单核细胞增生李斯特菌（LM）的小鼠会产生受H2-M3wt限制的CD8效应细胞，这些细胞识别由巨噬细胞呈递的热灭活LM相关抗原（HAA）。为了表征HAA，我们使用SDS或NaOH从LM中提取了一种生物活性成分。提取的HAA在亲水性溶剂中聚集，但在SDS存在下解离成较小的亚基，该亚基在Sephadex G-200中迁移，介于胰凝乳蛋白酶原（25 kDa）和细胞色素c（12.5 kDa）之间。在几乎降解所有可检测蛋白质的条件下，HAA的生物活性和大小不受蛋白酶K的影响。HAA也不受其他蛋白酶、核糖核酸酶和脱氧核糖核酸酶的影响，但HAA的生物活性被高碘酸盐破坏，高碘酸盐是一种降解碳水化合物的试剂。这些研究表明，H2-M3wt可以向CD8 T细胞呈递一种疏水性、非肽类的微生物抗原，其来源可能是糖脂。
