Lange H, Lemke H
Biochemisches Institut de Medizinischen Fakultät de Christian-Albrechts-Universität zu Kiel, Germany.
Int Immunol. 1996 May;8(5):683-8. doi: 10.1093/intimm/8.5.683.
B lymphocyte antigen receptors form an internal idiotypic network which is also connected by idiotypic interactions with the T lymphocyte compartment. Idiotypic-anti-idiotypic activation of lymphocytes has mainly been measured at the cellular level while the kinetics of primary anti-idiotypic humoral responses has so far not been determined. Here, we describe the induction of an anti-idiotypic immune response to the major idiotype (IdOx1) of the primary immune response in BALB/c mice to the hapten 2-phenyl-t-oxazolone coupled to chicken serum albumin. A primary anti-idiotypic humoral response could be induced with the phOx-binding and IdOx1-expressing, germline-encoded antibody H11.5 (mu, kappa) coupled to keyhole limpet hemocyanin. Compared to that of conventional antigens, the anti-idiotypic response showed a lag phase of 3 weeks. When the anti-IdOx1 serum titers had declined to background levels, a secondary anti-IdOx1 response could be induced even with soluble H11.5. This response showed as fast an increase as conventional antigens, but the antibody plateau did not exceed that of the primary response. During this secondary anti-IdOx1 response and probably to a small extent also during the primary response, the mice developed an idiotypically non-related IgM-anti-phOx response. In contrast, soluble H11.5 - either passively injected or transiently expressed during the early primary anti-hapten response - suppressed the anti-idiotypic response to H11.5 for up to 7 months in the majority of mice, while individual mice exhibited an early release from this suppression at various times. The differences and similarities between external and internal antigen-induced immune responses as well as the implications for idiotypic network regulation are discussed.
B淋巴细胞抗原受体形成一个独特型内部网络，该网络也通过独特型相互作用与T淋巴细胞区室相连。淋巴细胞的独特型-抗独特型激活主要在细胞水平上进行测量，而原发性抗独特型体液反应的动力学至今尚未确定。在此，我们描述了在BALB/c小鼠中，针对与鸡血清白蛋白偶联的半抗原2-苯基-对恶唑酮的原发性免疫反应的主要独特型（IdOx1）诱导的抗独特型免疫反应。可以用与钥孔血蓝蛋白偶联的、结合phOx并表达IdOx1的种系编码抗体H11.5（μ，κ）诱导原发性抗独特型体液反应。与传统抗原相比，抗独特型反应显示出3周的延迟期。当抗IdOx1血清滴度降至背景水平时，即使使用可溶性H11.5也能诱导继发性抗IdOx1反应。该反应的增加速度与传统抗原一样快，但抗体平台期不超过原发性反应。在这种继发性抗IdOx1反应期间，可能在原发性反应期间也有小程度的反应，小鼠产生了独特型不相关的IgM-抗phOx反应。相比之下，可溶性H11.5——无论是被动注射还是在早期原发性抗半抗原反应期间短暂表达——在大多数小鼠中可抑制对H11.5的抗独特型反应长达7个月，而个别小鼠在不同时间表现出对这种抑制的早期解除。讨论了外部和内部抗原诱导的免疫反应之间的差异和相似性以及对独特型网络调节的影响。
