Shostak A, Pivnik K, Gotloib L
Department of Nephrology, Central Emek Hospital, Afula, Israel.
Nephrol Dial Transplant. 1996 Apr;11(4):608-13. doi: 10.1093/oxfordjournals.ndt.a027348.
This study was designed to evaluate cytotoxic effects and influence upon cell growth of cultured mesothelial cells exposed to modified 4.25% Dianeal dialysate fluid (M-199 in Dianeal solution, glucose 4.25 g, pH 5.2) (Expr. group), 60 min a day for a total period of follow-up of 13 consecutive days, compared with that observed in a control group (C). Beginning on day 7, the cell counts in group C were significantly higher than those observed at zero time (P < 0.05). Cell counts in the experimental group showed no significant differences between the first day of culture and each one of the 13 consecutive days of follow-up. Thymidine incorporation into DNA observed on the first day in C, was significantly higher (P < 0.01) beginning on the 10th day. Values observed in the experimental group were low during the whole period of follow-up. LDH mean values at each time interval, were significantly higher (P < 0.01 and < 0.001) for cells exposed to the dialysis solution. Repeated exposure of the mesothelium to 40 mMol/l lactate and high glucose concentrations induced severe cell injury and death, decreased cell growth and, consequently, a reduced rate of regeneration which is extended as long as the repeated exposure is maintained.
本研究旨在评估暴露于改良的4.25% Dianeal透析液（Dianeal溶液中的M - 199，葡萄糖4.25 g，pH 5.2）（实验组）的培养间皮细胞的细胞毒性作用及其对细胞生长的影响，每天暴露60分钟，连续随访13天，并与对照组（C）进行比较。从第7天开始，C组的细胞计数显著高于零时观察到的细胞计数（P < 0.05）。实验组的细胞计数在培养第一天与连续13天随访中的每一天之间均无显著差异。C组在第一天观察到的DNA中胸苷掺入量，从第10天开始显著更高（P < 0.01）。实验组在整个随访期间观察到的值都很低。对于暴露于透析液的细胞，每个时间间隔的乳酸脱氢酶平均值显著更高（P < 0.01和< 0.001）。间皮反复暴露于40 mmol/l乳酸和高葡萄糖浓度会导致严重的细胞损伤和死亡，细胞生长减少，因此再生率降低，只要维持反复暴露，再生率就会持续降低。
