Takeda K, Nakamoto M, Yasunaga C, Nishihara G, Matsuo K, Urabe M, Goya T
Kidney Center, Saiseikai Yahata Hospital, Kitakyushu, Japan.
Adv Perit Dial. 1996;12:11-4.
In order to investigate which component of dialysate is responsible for mesothelial cell damage, we studied the chronic cytotoxicity to mesothelial cells by using four types of solution. Four different types of solution (Ringer's solution, lactate, high-concentrate glucose, and 4.25% Dianeal) were prepared and injected into the abdominal cavity of five ddY-mice, 5 mL every day for one month. The monolayer of the mesothelial cells was carefully removed from the liver surface by the imprint technique. Then, the mesothelial cell damage was evaluated by using trypan blue staining and comparing the results. Trypan blue-stained cells in 100 mm2 were observed in 7.0% of the control, 15.4% of the Ringer's solution, 18.7% of the lactate, 23.2% of the high-concentrate glucose, and 22.4% of the 4.25% Dianeal group, respectively. The damaged cells were significantly (p < 0.05) greater in the 4.25% Dianeal group than in the control group, and those in the high-concentrate glucose group also appeared to be greater in number compared to the control group It is concluded from these results that damage to the mesothelial cells is induced by chronic exposure to high-glucose dialysate, and its pathogenesis is deeply related to high concentration of glucose.
为了研究透析液的哪个成分导致间皮细胞损伤，我们使用四种溶液研究了对间皮细胞的慢性细胞毒性。制备了四种不同类型的溶液（林格氏液、乳酸盐、高浓度葡萄糖和4.25%的艾考糊精），并将其注入五只ddY小鼠的腹腔，每天5毫升，持续一个月。通过印记技术小心地从肝脏表面移除间皮细胞单层。然后，使用台盼蓝染色并比较结果来评估间皮细胞损伤。在对照组、林格氏液组、乳酸盐组、高浓度葡萄糖组和4.25%的艾考糊精组中，每100平方毫米中台盼蓝染色细胞的比例分别为7.0%、15.4%、18.7%、23.2%和22.4%。4.25%的艾考糊精组中受损细胞明显（p<0.05）多于对照组，高浓度葡萄糖组中的受损细胞数量与对照组相比似乎也更多。从这些结果可以得出结论，长期暴露于高糖透析液会导致间皮细胞损伤，其发病机制与高浓度葡萄糖密切相关。
