Moshier A, Reddy M S, Scannapieco F A
Department of Oral Biology, 318 Foster Hall, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Curr Microbiol. 1996 Sep;33(3):200-8. doi: 10.1007/s002849900100.
Saliva is known to modulate the adhesion of bacteria in the oral cavity. The present work was performed to assess the effect of salivary components on the adhesion of Escherichia coli to a model oral surface. Several genetically engineered E. coli strains were used to examine the role of type 1 fimbriation in the interaction of these strains with salivary components in solution or adsorbed to hydroxyapatite. High (MG1) and low (MG2) molecular weight salivary mucins, and secretory immunoglobulin A (sIgA), were found to interact with the surface of E. coli, and these interactions were independent of the expression of fimbriae or capsule. In contrast, fimbriated strains of E. coli adhered to a greater extent to saliva-coated synthetic hydroxyapatite (HAP) than did nonfimbriated strains. Testing of salivary components separated by gel filtration chromatography revealed that only high-molecular-weight components promoted adhesion of E. coli to HAP. Additional studies found that purified MG2 and sIgA promoted the adhesion of E. coli to HAP. Expression of type 1 fimbriae enhanced adhesion, while mannose inhibited adhesion of fimbriated strains, to saliva-coated HAP and to HAP coated with MG2 and sIgA. We conclude that salivary MG2 and sIgA may provide receptors for the adhesion of type 1 fimbriated E. coli to oral surfaces.
众所周知，唾液可调节口腔中细菌的黏附。开展本研究以评估唾液成分对大肠杆菌黏附于口腔模型表面的影响。使用了几种基因工程大肠杆菌菌株来研究1型菌毛在这些菌株与溶液中或吸附在羟基磷灰石上的唾液成分相互作用中的作用。发现高分子量（MG1）和低分子量（MG2）唾液黏蛋白以及分泌型免疫球蛋白A（sIgA）与大肠杆菌表面相互作用，且这些相互作用与菌毛或荚膜的表达无关。相比之下，有菌毛的大肠杆菌菌株比无菌毛的菌株更易黏附于唾液包被的合成羟基磷灰石（HAP）。对经凝胶过滤色谱分离的唾液成分进行检测发现，只有高分子量成分能促进大肠杆菌黏附于HAP。进一步研究发现，纯化的MG2和sIgA能促进大肠杆菌黏附于HAP。1型菌毛的表达增强了黏附，而甘露糖抑制了有菌毛菌株对唾液包被的HAP以及包被有MG2和sIgA的HAP的黏附。我们得出结论，唾液MG2和sIgA可能为1型有菌毛大肠杆菌黏附于口腔表面提供受体。
