Gabou L, Boisnard M, Gourdou I, Jammes H, Dulor J P, Djiane J
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
J Mol Endocrinol. 1996 Feb;16(1):27-37. doi: 10.1677/jme.0.0160027.
cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid sequence of the cloned prolactin cDNA presented a 93-78% identity with mink, porcine and human prolactins. The prolactin gene transcription was investigated by RT-PCR analysis in several organs of midlactating New Zealand White rabbits. The ectopic transcription of the prolactin gene was examined in more detail in the mammary gland. A strong PCR signal was detected in the mammary gland of virgin does and was also observed during pregnancy and at the beginning of lactation. This PCR signal was very weak in mid-lactating and absent in post-weaning mammary gland.
使用大鼠催乳素RNA探针从垂体文库中分离出编码兔催乳素的cDNA克隆。一个cDNA包含873个碱基，包括兔催乳素的完整编码序列、其信号肽以及分别为44和145个核苷酸的5'和3'非翻译区。克隆的催乳素cDNA推导的氨基酸序列与水貂、猪和人催乳素的同源性为93%-78%。通过RT-PCR分析研究了新西兰白兔泌乳中期几个器官中催乳素基因的转录情况。在乳腺中更详细地检测了催乳素基因的异位转录。在未孕母兔的乳腺中检测到强PCR信号，在怀孕期和泌乳初期也观察到该信号。该PCR信号在泌乳中期非常弱，在断奶后乳腺中不存在。
