Bansal N S
Molecular Microbiology Laboratory, NSW Health Department, Lidcombe, Australia.
Lett Appl Microbiol. 1996 May;22(5):353-6. doi: 10.1111/j.1472-765x.1996.tb01177.x.
Species-specific oligonucleotide primers were selected from the coding region of the listeriolysin O gene of Listeria monocytogenes and were used in conjunction with genus-specific primers and an internal control fragment for polymerase chain reaction amplification. The specificity of the primers was confirmed by testing 40 isolates of L. monocytogenes, other Listeria species and other micro-organisms which are ubiquitous in the environment. The reliability of these primers was further tested in parallel with standard cultural methods. In a preliminary study, over 250 different food samples were examined and PCR results were in complete agreement with those obtained from standard cultural procedures.
从单核细胞增生李斯特菌的溶血素O基因编码区选择种特异性寡核苷酸引物，并与属特异性引物和用于聚合酶链反应扩增的内部对照片段一起使用。通过检测40株单核细胞增生李斯特菌、其他李斯特菌属菌种以及环境中普遍存在的其他微生物，确认了引物的特异性。这些引物的可靠性与标准培养方法同时进行了进一步测试。在一项初步研究中，检测了250多个不同的食品样本，PCR结果与标准培养程序获得的结果完全一致。
