一种针对李斯特菌属中单核细胞增生李斯特菌的DNA探针。
A DNA probe specific for L. monocytogenes in the genus Listeria.
作者信息
Chenevert J, Mengaud J, Gormley E, Cossart P
机构信息
Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco.
出版信息
Int J Food Microbiol. 1989 Jul;8(4):317-9. doi: 10.1016/0168-1605(89)90002-0.
PMID:2518322
Abstract
Two DNA fragments encoding part of the listeriolysin O of Listeria monocytogenes have been used as probes in Southern blot experiments to detect the presence of the gene in the genus Listeria. Under stringent conditions, and using a fragment internal to the gene, only L. monocytogenes reacted with the probe.
摘要
编码单核细胞增生李斯特菌溶血素O部分序列的两个DNA片段已被用作Southern印迹实验中的探针，以检测李斯特菌属中该基因的存在。在严格条件下，使用基因内部的一个片段作为探针，只有单核细胞增生李斯特菌与该探针发生反应。
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