Loncarevic S, Tham W, Danielsson-Tham M L
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Lett Appl Microbiol. 1996 May;22(5):381-4. doi: 10.1111/j.1472-765x.1996.tb01184.x.
Restriction enzyme analysis (REA) with pulsed-field gel electrophoresis (PFGE) has been used to characterize and compare Listeria monocytogenes strains isolated from foods by two methods, an enrichment procedure and a direct plating procedure. In total 151 isolates from nine foods were investigated. In six of the foods (101 strains investigated) only one clone of L. monocytogenes was found irrespective of the method used. In three foods (50 strains investigated) the direct plating procedure yielded more clones than the enrichment procedure. At the most, five clones were detected in the same food. The results presented here indicate that direct plating from the food reveals more L. monocytogenes clones than revealed by an enrichment procedure.
采用脉冲场凝胶电泳（PFGE）进行的限制性内切酶分析（REA）已通过两种方法用于鉴定和比较从食品中分离出的单核细胞增生李斯特菌菌株，这两种方法分别是富集程序和直接平板接种程序。总共对来自九种食品的151株分离株进行了研究。在六种食品中（共研究了101株菌株），无论采用何种方法，均仅发现了单核细胞增生李斯特菌的一个克隆。在三种食品中（共研究了50株菌株），直接平板接种程序比富集程序产生了更多的克隆。在同一食品中最多检测到五个克隆。此处给出的结果表明，直接从食品中进行平板接种所揭示的单核细胞增生李斯特菌克隆比富集程序所揭示的更多。
