瑟氏泰勒虫梨形虫中性糖鞘脂的分析

Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms.

作者信息

Watarai S, Sugimoto C, Onuma M, Kobayashi K, Yasuda T

机构信息

Department of Cell Chemistry, Okayama University Medical School, Japan.

出版信息

J Vet Med Sci. 1996 Feb;58(2):165-8. doi: 10.1292/jvms.58.165.

DOI:10.1292/jvms.58.165

PMID:8672589

Abstract

Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC. N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively, N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Gal beta 1-4Glc beta 1-1Cer) and galactosylparagloboside (Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer), respectively.

摘要

从瑟氏泰勒虫梨形虫的脂质提取物中分离出中性糖鞘脂（GSLs），并通过薄层色谱法（TLC）和脂质体免疫裂解试验（LILA）进行分析；在TLC上分离出两种主要的GSLs，分别命名为N-1和N-2。N-1 GSL在TLC板上的迁移率与乳糖基神经酰胺（LacCer）相同。另一方面，N-2 GSL在TLC板上的迁移率与半乳糖基副球蛋白相同。为了鉴定瑟氏泰勒虫中性GSLs的分子种类，分别用抗LacCer和半乳糖基副球蛋白的抗体通过LILA对N-1和N-2 GSLs进行检测，N-1 GSL与抗LacCer抗体有反应，N-2与抗半乳糖基副球蛋白抗体有反应。这些结果表明，N-1和N-2 GSLs分别是乳糖基神经酰胺（Galβ1-4Glcβ1-1Cer）和半乳糖基副球蛋白（Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer）。