Cserháti T, Forgács E
Central Research Institute for Chemistry, Budapest, Hungary.
J Chromatogr A. 1996 Mar 29;728(1-2):67-73. doi: 10.1016/0021-9673(95)01015-7.
The retention parameters of eighteen commercial anticancer drugs were determined on a beta-cyclodextrin polymer-coated silica support (beta CDP) using methanol-water mixtures as eluent and the relationship between the retention behaviour and physico-chemical parameters was elucidated by principal component analysis (PCA) followed by two-dimensional non-linear mapping. No significant linear correlation was found between the retention behaviour of drugs on octadecylsilica and beta CDP silica columns, indicating that the retention capacity and selectivity of the columns are considerably different. The results of PCA indicated that hydrophobic and electronic interactions and steric conditions govern the retention of anticancer drugs on beta CDP column, suggesting a mixed retention mechanism.
使用甲醇 - 水混合物作为洗脱剂，在β-环糊精聚合物包覆的硅胶载体（βCDP）上测定了18种市售抗癌药物的保留参数，并通过主成分分析（PCA）和二维非线性映射阐明了保留行为与物理化学参数之间的关系。未发现药物在十八烷基硅胶柱和βCDP硅胶柱上的保留行为之间存在显著的线性相关性，这表明这两种柱的保留容量和选择性有很大差异。PCA结果表明，疏水和电子相互作用以及空间条件决定了抗癌药物在βCDP柱上的保留，提示存在混合保留机制。
