Cserháti T, Forgács E
Central Research Institute of Chemistry, Hungarian Academy of Sciences, Budapest, Hungary.
J Chromatogr B Biomed Appl. 1996 May 31;681(1):205-11. doi: 10.1016/0378-4347(95)00469-6.
The retention of eighteen steroid drugs was determined on a beta-cyclodextrin polymer (beta CDP)-coated silica column using methanol-water mixtures as eluents. The relative strength of inclusion formation between the drugs and hydroxypropyl-beta CD (HP beta CD) and dimethyl-beta CD was determined by charge transfer chromatography carried out on reversed-phase thin-layer chromatography plates. The retention characteristics of drugs were correlated with their physicochemical parameters and with their inclusion complex-forming capacity. Calculations indicated that the inclusion complex-forming capacity of the drugs has little impact on the retention that is due to the HP beta CD and water-insoluble beta CD polymers exposed to different retention characteristics. The hydrophilic molecular parameters of drugs significantly influenced their retention. This result suggests that the selectivity of the beta CDP-coated column may be different from that of the traditional reversed-phase columns.
使用甲醇 - 水混合物作为洗脱剂，在β - 环糊精聚合物（βCDP）涂覆的硅胶柱上测定了18种甾体药物的保留情况。通过在反相薄层色谱板上进行的电荷转移色谱法，测定了药物与羟丙基 - β环糊精（HPβCD）和二甲基 - β环糊精之间包合物形成的相对强度。药物的保留特性与其物理化学参数及其包合物形成能力相关。计算表明，由于HPβCD和具有不同保留特性的水不溶性βCD聚合物，药物的包合物形成能力对保留的影响很小。药物的亲水性分子参数显著影响其保留。该结果表明，βCDP涂覆柱的选择性可能与传统反相柱的选择性不同。
