Kruszewska D, Tylewska-Wierzbanowska S
National Institute of Hygiene, Warsaw, Poland.
Rocz Akad Med Bialymst. 1996;41(1):129-35.
Ticks Ixodes ricinus have been harvested in park down-town Wałcz in June 1994. Presence of Borrelia burgdorferi and rickettsiae has been tested with PCR. B. burgdorferi and Coxiella burnetii have not been detected in any tick whereas characteristic for other rickettsiae DNA fragment of gene encoding citrate-synthase has been found. Bacterial strain has been cultured from the tick. Biochemical properties of isolated strain has strongly suggested that these bacteria belong to genus Bartonella (Rochalimaea). Further identification of bacterial DNA with RFLP-PCR (restriction fragment length polymorphism-PCR) has shown characteristics of Bartonella bacilliformis species not recognised in Poland until recently.
1994年6月，在瓦尔奇市中心公园采集到蓖麻硬蜱。采用聚合酶链反应（PCR）检测了伯氏疏螺旋体和立克次氏体的存在情况。在任何蜱中均未检测到伯氏疏螺旋体和贝氏柯克斯体，而发现了编码柠檬酸合酶的基因的其他立克次氏体DNA片段特征。从蜱中培养出了细菌菌株。分离菌株的生化特性强烈表明这些细菌属于巴尔通体属（罗沙利马体属）。用限制性片段长度多态性聚合酶链反应（RFLP-PCR）对细菌DNA进行进一步鉴定，结果显示出直到最近在波兰还未被识别的杆状巴尔通体物种的特征。
