一种竞争性酶免疫测定法用于梅毒筛查的评估。
Evaluation of a competitive enzyme immunoassay in screening for syphilis.
作者信息
De Majo E, Bianchini G, Parri F, Tocci E, Monaci M, Paoli C
机构信息
Laboratorio di Batteriologia e Virologia, USL/10D, Florence, Italy.
出版信息
New Microbiol. 1996 Jan;19(1):31-8.
A competitive immunoenzymatic method has been developed and evaluated for the serological screening of syphilis. The method detects both anti-Treponema Pallidum IgG and IgM. The kit, commercially known as "Syphilis Screen", is produced by DIESSE Diagnostica Senese (Siena, Italy); all the required reagents are included and are ready for use. The test is performed on undiluted serum and a single incubation step is necessary. The method can be easily automated, and the results do not require a subjective interpretation. A good correlation was found with the Treponema Pallidum Haemagglutination (TPHA) technique: only 14 out of 2350 samples tested (2090 non reactive and 260 reactive) were found to be in disagreement. This test can be considered an alternative to the TPHA method in screening for syphilis.
已开发并评估了一种用于梅毒血清学筛查的竞争性免疫酶法。该方法可检测抗梅毒螺旋体IgG和IgM。该试剂盒商业名称为“梅毒筛查”，由迪塞诊断公司（意大利锡耶纳）生产；包含所有所需试剂，可立即使用。该检测在未稀释的血清上进行，只需一个孵育步骤。该方法可轻松实现自动化，结果无需主观解读。与梅毒螺旋体血凝试验（TPHA）技术有良好的相关性：在2350份检测样本中（2090份无反应性和260份有反应性），仅发现14份结果不一致。该检测可被视为梅毒筛查中TPHA方法的替代方法。
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