Pelloux H, Weiss J, Simon J, Muet F, Fricker-Hidalgo H, Goullier-Fleuret A, Ambroise-Thomas P
Département de Parasitologie-Myocologie Médicale et Moléculaire, Faculté de Médecine, Université Joseph Fourier, Grenoble, France.
FEMS Microbiol Lett. 1996 Apr 15;138(1):11-5. doi: 10.1111/j.1574-6968.1996.tb08127.x.
A new PCR system including a pair of primers, a probe and an internal control were designed from the B1 gene of Toxoplasma gondii. The system described allowed the detection of less than 10 tachyzoites of the RH strain of T. gondii. Among 21 amniotic fluid samples, this system diagnosed the cases of congenital toxoplasmosis which were simultaneously diagnosed using mice inoculation, in vitro culture, and serology from both amniotic fluid and fetal blood. These results show that these new primers allow for a highly sensitive detection of T. gondii DNA.
一种新的聚合酶链式反应（PCR）系统，包括一对引物、一个探针和一个内部对照，是根据刚地弓形虫的B1基因设计的。所述系统能够检测到少于10个刚地弓形虫RH株速殖子。在21份羊水样本中，该系统诊断出的先天性弓形虫病病例，与同时采用小鼠接种、体外培养以及羊水和胎儿血液血清学检测诊断出的病例一致。这些结果表明，这些新引物能够高度灵敏地检测刚地弓形虫DNA。
