Gudelj L, Deniz G, Rukavina D, Johnson P M, Christmas S E
Department of Immunology, University of Liverpool, UK.
Immunology. 1996 Apr;87(4):609-15. doi: 10.1046/j.1365-2567.1996.508583.x.
Cell surface and cytoplasmic antigen expression by 35 CD3- decidual granular leucocyte (DGL) clones, derived from human endometrial tissue in the first trimester of pregnancy, has been compared with both that of fresh CD3- decidual leucocytes and that of CD3- peripheral blood natural killer (PBNK) cell clones (n = 12). The majority of DGL clones retained the antigenic phenotype of fresh cells, although CD103 (HML-1) was expressed on 50% of DGL clones but only 17% of fresh DGL. Both cytoplasmic CD3 zeta and CD3 epsilon chains were detected in > 90% of DGL clones in the absence of cell surface CD3. Cytoplasmic CD3 zeta was present in almost all fresh CD3- DGL, whereas CD3 epsilon was not. Most DGL clones did not express surface Fc gamma receptors I-III (CD64, -32 and -16, respectively) and complement receptors (CR) types 1 and 2 (CD35 and 21, respectively), but 43% expressed CR3 (CD11b/18); in contrast, all PBNK clones were CR3+. The NK cell-associated molecules Kp43 (CD94) and the p58 molecule recognized by the HP3E4 monoclonal antibody were both present on a higher proportion of CD3- PBNK (91% and 50%, respectively) than DGL clones (31% and 14%, respectively), despite expression of CD94 by > 90% of fresh CD56+ decidual leucocytes. Five of 35 CD3- DGL clones expressed cytoplasmic CD3 zeta in the absence of expression of CD2, CD16 or the p58 molecule recognized by HP3E4. These variations between CD3- DGL and PBNK cell clones in expression of functional molecules may be related to previously reported differences in major histocompatibility complex-non-restricted cytotoxic activities between these two cell types.
从妊娠头三个月的人子宫内膜组织中获得的35个CD3阴性蜕膜颗粒白细胞（DGL）克隆的细胞表面和细胞质抗原表达，已与新鲜的CD3阴性蜕膜白细胞以及CD3阴性外周血自然杀伤（PBNK）细胞克隆（n = 12）的抗原表达进行了比较。大多数DGL克隆保留了新鲜细胞的抗原表型，尽管CD103（HML-1）在50%的DGL克隆中表达，但在新鲜DGL中仅为17%。在没有细胞表面CD3的情况下，> 90%的DGL克隆中检测到细胞质CD3 ζ链和CD3 ε链。几乎所有新鲜的CD3阴性DGL中都存在细胞质CD3 ζ，而CD3 ε则不存在。大多数DGL克隆不表达表面Fcγ受体I-III（分别为CD64、-32和-16）和补体受体（CR）1型和2型（分别为CD35和21），但43%表达CR3（CD11b/18）；相比之下，所有PBNK克隆均为CR3阳性。NK细胞相关分子Kp43（CD94）和被HP3E4单克隆抗体识别的p58分子在CD3阴性PBNK中的比例（分别为91%和50%）均高于DGL克隆（分别为31%和14%），尽管> 90%的新鲜CD56阳性蜕膜白细胞表达CD94。35个CD3阴性DGL克隆中有五个在不表达CD2、CD16或被HP3E4识别的p58分子的情况下表达细胞质CD3 ζ。CD3阴性DGL和PBNK细胞克隆在功能分子表达上的这些差异可能与先前报道的这两种细胞类型之间主要组织相容性复合体非限制性细胞毒性活性的差异有关。
