Ferguson S R, Borchman D, Yappert M C
Department of Chemistry, Kentucky Lions Eye Research Institute, University of Louisville 40292, USA.
Invest Ophthalmol Vis Sci. 1996 Jul;37(8):1703-6.
To confirm the identity of the major component of the human lens membranes proposed in 1994 to be dihydrosphingomyelin (DH-SPH).
DH-SPH was prepared by catalytic hydrogenation of the double bond between carbons 4 and 5 of sphingomyelin (SPH). DH-SPH was characterized by phosphorus-31 (31P) and proton (1H) nuclear magnetic resonance (NMR) spectroscopy at different reaction times. The spectroscopic data were compared to those of the major component extracted from human lens membranes.
Both the 1H NMR and the 31P NMR spectral resonances of the prepared DH-SPH matched those for the once "unknown phospholipid" that constitutes approximately half the human lens phospholipids.
The match of the spectroscopic NMR data obtained for the DH-SPH prepared by hydrogenation of SPH and those for the major phospholipid isolated from the human lens membranes confirms the identity of this sphingolipid as D-erythro-4,5-dihydrosphingomyelin.
确认1994年提出的人晶状体膜主要成分二氢鞘磷脂（DH-SPH）的身份。
通过对鞘磷脂（SPH）碳4和碳5之间的双键进行催化氢化制备DH-SPH。在不同反应时间通过磷-31（31P）和质子（1H）核磁共振（NMR）光谱对DH-SPH进行表征。将光谱数据与从人晶状体膜中提取的主要成分的数据进行比较。
所制备的DH-SPH的1H NMR和31P NMR光谱共振与构成约一半人晶状体磷脂的曾经“未知磷脂”的光谱共振相匹配。
通过SPH氢化制备的DH-SPH所获得的NMR光谱数据与从人晶状体膜中分离的主要磷脂的光谱数据相匹配，证实了这种鞘脂为D-赤藓糖型-4,5-二氢鞘磷脂。
