Yadav B S, Rani U, Dhamija S S, Nigam P, Singh D
Department of Microbiology, CCS Haryana Agricultural University, Hisar, India.
J Basic Microbiol. 1996;36(3):205-10. doi: 10.1002/jobm.3620360307.
Continuous ethanol production by immobilized cells of Saccharomyces cerevisiae HAU-1 has been studied using synthetic and molasses based medium in column reactors. Immobilization of 30% yeast cells biomass (wet) in 1.5% calcium alginate gel resulted in the production of 20.8 g. l-1.h-1 alcohol at a dilution rate of 0.36 h-1 with approximately 1/3rd volume of the column reactor packed with gel beads. Optimum diameter of the beads was found to be 3.5 mm for efficient fermentation. The size of the column reactor (length to diameter ratio) also affected the productivity and fermentation efficiency due to gas hold-up and mass transfer effects. Molasses could also be fermented by this system but at a lower fermentation efficiency which could be improved, to some extent, by supplementation with nutrients.
利用固定化的酿酒酵母HAU-1细胞，在柱式反应器中使用合成培养基和基于糖蜜的培养基进行了连续乙醇生产的研究。将30%的酵母细胞生物质（湿重）固定在1.5%的海藻酸钙凝胶中，在稀释率为0.36 h⁻¹时，以约1/3体积的柱式反应器填充凝胶珠，可产生20.8 g·L⁻¹·h⁻¹的酒精。发现珠子的最佳直径为3.5 mm以实现高效发酵。由于气体滞留和传质效应，柱式反应器的尺寸（长径比）也会影响生产率和发酵效率。该系统也可以发酵糖蜜，但发酵效率较低，通过补充营养物质可在一定程度上提高发酵效率。
