Doronin S V, Hemenway C
Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27695-7622, USA.
J Virol. 1996 Jul;70(7):4795-9. doi: 10.1128/JVI.70.7.4795-4799.1996.
Membrane-containing extracts isolated from tobacco plants infected with the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-weight PVX RNA products (R1 to R4). Nuclease digestion and hybridization studies indicated that R1 and R2 are a mixture of partially single-stranded replicative intermediates and double-stranded replicative forms. R3 and R4 are double-stranded products containing sequences typical of the two major PVX subgenomic RNAs. The newly synthesized RNAs were demonstrated to have predominantly plus-strand polarity. Synthesis of these products was remarkably stable in the presence of ionic detergents.
从感染正链RNA病毒马铃薯X病毒（PVX）的烟草植株中分离得到的含膜提取物，支持四种主要的高分子量PVX RNA产物（R1至R4）的合成。核酸酶消化和杂交研究表明，R1和R2是部分单链复制中间体和双链复制形式的混合物。R3和R4是双链产物，包含两种主要PVX亚基因组RNA的典型序列。新合成的RNA被证明主要具有正链极性。在离子去污剂存在的情况下，这些产物的合成非常稳定。
