马铃薯 X 病毒编码的 RNA 依赖的 RNA 聚合酶的 N 端α螺旋对于膜结合和多聚化是必需的。
The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization.
机构信息
College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, China.
出版信息
Viruses. 2022 Aug 28;14(9):1907. doi: 10.3390/v14091907.
Positive-sense single-stranded RNA viruses replicate in virus-induced membranous organelles for maximum efficiency and immune escaping. The replication of potato virus X (PVX) takes place on the endoplasmic reticulum (ER); however, how PVX-encoded RNA-dependent RNA polymerase (RdRp) is associated with the ER is still unknown. A proline-kinked amphipathic α-helix was recently found in the MET domain of RdRp. In this study, we further illustrate that the first α-helix of the MET domain is also required for ER association. Moreover, we found that the MET domain forms multimers on ER and the first α-helix is essential for multimerization. These results suggest that the RdRp of PVX adopts more than one hydrophobic motif for membrane association and for multimerization.
正链单链 RNA 病毒在病毒诱导的膜细胞器中复制，以达到最高效率和免疫逃避。马铃薯 X 病毒 (PVX) 的复制发生在内质网上;然而，PVX 编码的 RNA 依赖性 RNA 聚合酶 (RdRp) 如何与内质网相关联尚不清楚。最近在 RdRp 的 MET 结构域中发现了一个脯氨酸扭曲的两亲性α-螺旋。在这项研究中，我们进一步说明，MET 结构域的第一个α-螺旋也需要与内质网结合。此外，我们发现 MET 结构域在 ER 上形成多聚体，并且第一个α-螺旋对于多聚化是必需的。这些结果表明，PVX 的 RdRp 采用了不止一种疏水基序来进行膜结合和多聚化。
Zotero 插件