Sasakuma F, Shimizu T, Ishikawa O, Taku K, Doi K, Kojima J
Department of Laboratory Medicine, Center for Adult Diseases, Osaka, Japan.
Rinsho Byori. 1996 May;44(5):471-6.
A rapid method for determining gastrin, quick gastrin, has been developed. Separation/washing procedure has been improved and can be completed within three minutes. It required only 48 minutes for the assay of 22 blood samples. Quick gastrin is a RIA that uses magnetic particles. On magnetic particles, a goat anti-rabbit IgG antibody is bound covalently. An anti-human gastrin rabbit antibody is bound to an anti-rabbit IgG antibody. Assay is started by adding the magnetic particles to a mixture of sample and 125I-gastrin. Following 30 minute incubation at 37 degrees C, the particles are sedimented in a magnetic field and washed. The gastrin content of the sample is then quantitated by counting radioactivity of the particles. Incomplete equilibration of antigen-antibody reaction is corrected using standard solution prepared from charcoal treated plasma. The immunoreactive gastrin values by quick gastrin correlated well with those by a commercial assay kit (Gammadab RIA kit; y = 1.01 x +4.3, r = 0.99). When compared to a reported conventional rapid assay, quick gastrin is easier and more accurate. Quick gastrin is sensitive enough to use for intra-operative determination of gastrin. We applied quick gastrin to the samples obtained from intra-operative secretin test in a gastrinoma patient. Twofold increases in gastrin after injection of secretin clearly indicated the existence of occult gastrinomas in her pancreas. When gastrin was assayed with the conventional rapid method, the increase in gastrin was less and did not reach the criteria for existence of gastrinoma.
一种用于测定胃泌素的快速方法——快速胃泌素法已被开发出来。分离/洗涤程序得到了改进，可在三分钟内完成。检测22份血样仅需48分钟。快速胃泌素是一种使用磁性颗粒的放射免疫分析法。在磁性颗粒上，山羊抗兔IgG抗体共价结合。抗人胃泌素兔抗体与抗兔IgG抗体结合。通过将磁性颗粒加入样品和125I-胃泌素的混合物中开始检测。在37℃孵育30分钟后，颗粒在磁场中沉淀并洗涤。然后通过计数颗粒的放射性来定量样品中的胃泌素含量。使用由经木炭处理的血浆制备的标准溶液校正抗原-抗体反应的不完全平衡。快速胃泌素法测得的免疫反应性胃泌素值与商业检测试剂盒（伽马达布放射免疫分析试剂盒；y = 1.01x + 4.3，r = 0.99）测得的值相关性良好。与报道的传统快速检测方法相比，快速胃泌素法更简便、更准确。快速胃泌素的灵敏度足以用于术中胃泌素的测定。我们将快速胃泌素法应用于一名胃泌素瘤患者术中促胰液素试验获得的样本。注射促胰液素后胃泌素增加两倍清楚地表明她的胰腺中存在隐匿性胃泌素瘤。当用传统快速方法检测胃泌素时，胃泌素的增加较少，未达到胃泌素瘤存在的标准。
