Sasakuma F, Hasegawa K, Ishikawa O, Yasuda T, Matumiya K, Kojima J
Department of Laboratory Medicine, Center for Adult Diseases, Osaka.
Rinsho Byori. 1990 Nov;38(11):1267-72.
A quick RIA for IRI was developed which can be applied for localizing occult insulinoma during extirpation of the tumor. We used anti-insulin antibody insolubilized with bacterial cell wall. The standard solutions were prepared by dissolving porcine insulin in human plasma containing low level of IRI. By use of this assay IRI in plasma samples are assayed within 45 min for 20 samples including standard samples. The coefficient of variation of intra- and inter-assay were 9-19% and the recovery tests were 70-120%. The assay has a sensitivity of 10 microU/ml. The obtained IRI values by this method correlated well with those by the usual method (Insulin RIA Bead) (y=1.0 x -0.2, r = 0.98). A clinical case with insulinoma was shown in which the localization of the tumor was diagnosed from IRI levels in various portions of splenic vein assayed by this method during the operation and the tumor was extirpated successfully.
我们开发了一种快速IRI放射免疫分析方法，可用于在肿瘤切除术中定位隐匿性胰岛素瘤。我们使用了与细菌细胞壁结合的抗胰岛素抗体。标准溶液是通过将猪胰岛素溶解在含有低水平IRI的人血浆中制备的。使用该分析方法，45分钟内可对包括标准样品在内的20个血浆样品进行IRI检测。批内和批间变异系数分别为9%-19%，回收率测试为70%-120%。该分析方法的灵敏度为10微单位/毫升。通过该方法获得的IRI值与常规方法（胰岛素放射免疫分析珠法）获得的值相关性良好（y=1.0x-0.2，r=0.98）。展示了一个胰岛素瘤临床病例，术中通过该方法检测脾静脉各部位的IRI水平诊断出肿瘤位置，并成功切除了肿瘤。
