AlDosari A, McDonald J, Olson B, Noblitt T, Li Y, Stookey G
Department of Oral Biology, Indiana University, School of Dentistry, Indianapolis 46202, USA.
Mutat Res. 1996 Jun 10;352(1-2):1-7. doi: 10.1016/0027-5107(96)00149-2.
The purpose of this study was to investigate the influence of benzylisothiocyanate (BIT) and 13-cis-retinoic acid (RA) upon the genotoxic potential of benzo[a]pyrene (BaP) to induce micronucleus formation in the bone marrow of mice. Eighty-two male mice were divided into 10 groups. One group served as a negative control (olive oil intubation). Four groups received an oral intubation of various concentrations of BIT (15 to 120 mg/kg) and i.p. injections of BaP (185 mg/kg). Another four groups were treated identically, but received RA (20 to 150 mg/kg) in place of BIT. Finally, one group received only i.p. injection of BaP (185 mg/kg). The results showed that both BIT and RA significantly reduced the frequency of micronucleus formation in the bone marrow of the BaP treated animals. BIT was found to be effective at all the tested concentrations. RA was effective only at three of the four tested concentrations (40, 75 and 150 mg/kg). These findings indicate that both BIT and RA may reduce the genotoxic effects of benzo[a]pyrene in the mice under the test conditions utilized.
本研究的目的是调查苄基异硫氰酸酯（BIT）和13 - 顺式维甲酸（RA）对苯并[a]芘（BaP）诱导小鼠骨髓细胞形成微核的遗传毒性潜力的影响。82只雄性小鼠被分为10组。一组作为阴性对照（腹腔注射橄榄油）。四组经口灌胃不同浓度的BIT（15至120毫克/千克）并腹腔注射BaP（185毫克/千克）。另外四组接受相同处理，但用RA（20至150毫克/千克）代替BIT。最后，一组仅腹腔注射BaP（185毫克/千克）。结果表明，BIT和RA均显著降低了BaP处理动物骨髓中微核形成的频率。在所有测试浓度下均发现BIT有效。RA仅在四个测试浓度中的三个（40、75和150毫克/千克）有效。这些发现表明，在所采用的测试条件下，BIT和RA均可降低苯并[a]芘对小鼠的遗传毒性作用。
