Jones J M, Sussman G L, Beezhold D H
C.R. Bard Inc., Covington, Georgia 30209, USA.
Urology. 1996 Jun;47(6):898-902. doi: 10.1016/S0090-4295(96)00037-4.
To assess the latex allergen content of glutaraldehyde cross-linked injectable bovine collagen stored in rubber plunger syringes.
Extracts of syringe plungers and collagen solutions before and after storage in syringes with natural rubber latex plungers were tested for latex protein allergens. Thirty-nine patients known to be allergic to latex underwent skin prick testing with extracts of the latex plungers, collagen solutions before and after storage in syringes, standard latex skin test reagents, four extracts from commercially available gloves, and positive (histamine) and negative (diluent) control solutions. Thirty-one control patients not known to be latex allergic were similarly tested.
No latex proteins were detected using in vitro immunochemical techniques. Only 1 of 39 (2.5%) latex allergic patients reacted to the syringe extract and the collagen stored in the syringe. No reactions were recorded to collagen that no contact with latex.
The level of latex antigens in injectable collagen is very low. The low prevalence of skin test reactivity in these highly latex allergic individuals suggests that type 1 hypersensitivity reactions as a result of latex contamination would be unlikely.
评估储存在橡胶活塞注射器中的戊二醛交联注射用牛胶原蛋白的乳胶过敏原含量。
对天然橡胶乳胶活塞注射器储存前后的注射器活塞提取物和胶原蛋白溶液进行乳胶蛋白过敏原检测。39名已知对乳胶过敏的患者用乳胶活塞提取物、注射器储存前后的胶原蛋白溶液、标准乳胶皮肤试验试剂、四种市售手套提取物以及阳性（组胺）和阴性（稀释剂）对照溶液进行皮肤点刺试验。31名未知对乳胶过敏的对照患者也进行了类似检测。
采用体外免疫化学技术未检测到乳胶蛋白。39名乳胶过敏患者中只有1名（2.5%）对注射器提取物和储存在注射器中的胶原蛋白有反应。未接触乳胶的胶原蛋白未记录到反应。
注射用胶原蛋白中的乳胶抗原水平非常低。在这些高度乳胶过敏个体中皮肤试验反应性的低发生率表明，因乳胶污染导致的I型超敏反应不太可能发生。
